Identification of a novel Bcl-2 related gene, BRAG-1, in human glioma

Rita Das, E. Premkumar Reddy, Devjani Chatterjee, David W. Andrews

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Programmed cell death (apoptosis) plays a major role in embryogenesis, in mature organ homeostasis and in many disease states including cancer. Apoptosis occurs as an orderly cell-intrinsic suicide program regulated by a family of genes related to Bcl-2. Here, we describe the cloning and molecular characterization of a gene homologous to Bcl-2 from a human glioma. This gene named BRAG-1 (for brain-related apoptosis gene) has an open reading frame that encodes for a protein of 31 kDa sharing significant sequence homology with the Bcl-2 family of genes in the BH1 and BH2 regions. Northern blot analyses revealed that BRAG-1 is expressed in human gliomas as a 1.8 kb message. This gene, interestingly, was found to be expressed predominantly in normal human brain as a 4.5 kb transcript which is different in size from the message found in tumor tissues. These results suggest that BRAG-1 may be rearranged in human gliomas leading to its over-expression as a truncated transcript. Utilizing a bacterial expression vector, we produced BRAG-1 protein which was found to cross-react with a Bcl-2 monoclonal antibody, further suggesting structural and immunological similarity to Bcl-2.

Original languageEnglish
Pages (from-to)947-951
Number of pages5
JournalOncogene
Volume12
Issue number5
StatePublished - 1996
Externally publishedYes

Keywords

  • Apoptosis
  • BRAG-1
  • Bcl-2
  • Glioma

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