TY - JOUR
T1 - Identification of a NIPSNAP homologue as host cell target for Salmonella virulence protein SpiC
AU - Lee, Aaron H.
AU - Zareei, Mahboubeh Papari
AU - Daefler, Simon
PY - 2002/11/1
Y1 - 2002/11/1
N2 - Salmonella enterica uses a type III secretion system encoded by SPI-2 to target specific virulence factors into the host cytosol of macrophages to inhibit the phagosomal-lysosomal maturation pathway. This ensures survival of Salmonella inside its intracellular niche, the Salmonella-containing vacuole (SCV). One such virulence factor is SpiC, which was previously shown to interfere with intracellular vesicular trafficking. In this study we have used a yeast two-hybrid assay to identify a NIPSNAP homologue as host cell target for SpiC that we have termed TassC. In vitro and in vivo co-purification of SpiC and TassC confirm the specificity of this interaction. Suppression of TassC production compensates a SpiC production deficit and allows spiC- Salmonella to survive within macrophages at levels comparable to wild-type Salmonella. We hypothesize that TassC is a host cell factor that determines vesicular trafficking in macrophages and is inactivated by Salmonella SpiC.
AB - Salmonella enterica uses a type III secretion system encoded by SPI-2 to target specific virulence factors into the host cytosol of macrophages to inhibit the phagosomal-lysosomal maturation pathway. This ensures survival of Salmonella inside its intracellular niche, the Salmonella-containing vacuole (SCV). One such virulence factor is SpiC, which was previously shown to interfere with intracellular vesicular trafficking. In this study we have used a yeast two-hybrid assay to identify a NIPSNAP homologue as host cell target for SpiC that we have termed TassC. In vitro and in vivo co-purification of SpiC and TassC confirm the specificity of this interaction. Suppression of TassC production compensates a SpiC production deficit and allows spiC- Salmonella to survive within macrophages at levels comparable to wild-type Salmonella. We hypothesize that TassC is a host cell factor that determines vesicular trafficking in macrophages and is inactivated by Salmonella SpiC.
UR - http://www.scopus.com/inward/record.url?scp=0036853636&partnerID=8YFLogxK
U2 - 10.1046/j.1462-5822.2002.00225.x
DO - 10.1046/j.1462-5822.2002.00225.x
M3 - Review article
C2 - 12427096
AN - SCOPUS:0036853636
SN - 1462-5814
VL - 4
SP - 739
EP - 750
JO - Cellular Microbiology
JF - Cellular Microbiology
IS - 11
ER -