Identification of a NIPSNAP homologue as host cell target for Salmonella virulence protein SpiC

Aaron H. Lee, Mahboubeh Papari Zareei, Simon Daefler

Research output: Contribution to journalReview articlepeer-review

52 Scopus citations

Abstract

Salmonella enterica uses a type III secretion system encoded by SPI-2 to target specific virulence factors into the host cytosol of macrophages to inhibit the phagosomal-lysosomal maturation pathway. This ensures survival of Salmonella inside its intracellular niche, the Salmonella-containing vacuole (SCV). One such virulence factor is SpiC, which was previously shown to interfere with intracellular vesicular trafficking. In this study we have used a yeast two-hybrid assay to identify a NIPSNAP homologue as host cell target for SpiC that we have termed TassC. In vitro and in vivo co-purification of SpiC and TassC confirm the specificity of this interaction. Suppression of TassC production compensates a SpiC production deficit and allows spiC- Salmonella to survive within macrophages at levels comparable to wild-type Salmonella. We hypothesize that TassC is a host cell factor that determines vesicular trafficking in macrophages and is inactivated by Salmonella SpiC.

Original languageEnglish
Pages (from-to)739-750
Number of pages12
JournalCellular Microbiology
Volume4
Issue number11
DOIs
StatePublished - 1 Nov 2002
Externally publishedYes

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