Identification of a cell lineage-specific gene coding for a sea urchin α2(IV)-like collagen chain

Jean Yves Exposito, Hiroaki Suzuki, Christope Geourjon, Robert Garrone, Michael Solursh, Francesco Ramirez

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

We report the isolation of several overlapping cDNAs from an embryonic library of Strongylocentrotus purpuratus coding for a novel sea urchin collagen chain. The conceptual amino acid translation of the cDNAs indicated that the protein displays the structural features of a vertebrate type IV- like collagen α chain. In addition to a putative 31-residue signal peptide, the sea urchin molecule contains a 14-residue amino-terminal non-collagenous segment, a discontinuous 1,477-amino acid triple helical domain, and a 225- residue carboxyl-terminal domain rich in cysteines. The amino- and carboxyl- terminal non-collagenous regions of the echinoid molecule are remarkably similar to the 7 S and carboxyl-terminal non-collagenous (NC1) domains of the α1 and α2 chains of vertebrate type IV collagen. The sequence similarity and distinct structural features of the 7 S and NC1 domains strongly suggest that the sea urchin polypeptide is evolutionarily related to the α2(IV) class of collagen chains. Finally, in situ hybridizations revealed that expression of this collagen gene is restricted to the mesenchyme cell lineage of the developing sea urchin embryo.

Original languageEnglish
Pages (from-to)13167-13171
Number of pages5
JournalJournal of Biological Chemistry
Volume269
Issue number18
StatePublished - 6 May 1994

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