TY - JOUR
T1 - Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents
AU - Laveriano-Santos, Emily P.
AU - Marhuenda-Muñoz, María
AU - Vallverdú-Queralt, Anna
AU - Martínez-Huélamo, Miriam
AU - Tresserra-Rimbau, Anna
AU - Miliarakis, Elefterios
AU - Arancibia-Riveros, Camila
AU - Jáuregui, Olga
AU - Ruiz-León, Ana María
AU - Castro-Baquero, Sara
AU - Estruch, Ramón
AU - Bodega, Patricia
AU - de Miguel, Mercedes
AU - de Cos-Gandoy, Amaya
AU - Martínez-Gómez, Jesús
AU - Santos-Beneit, Gloria
AU - Fernández-Alvira, Juan M.
AU - Fernández-Jiménez, Rodrigo
AU - Lamuela-Raventós, Rosa M.
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/6
Y1 - 2022/6
N2 - This study aimed to develop and validate a liquid chromatography/electrospray ionization-linear ion trap quadrupole-Orbitrap-high-resolution mass spectrometry (HPLC/ESI-LTQ-Orbitrap-HRMS) method to identify and quantify urinary microbial phenolic metabolites (MPM), as well as to explore the relationship between MPM and dietary (poly)phenols in Spanish adolescents. A total of 601 spot urine samples of adolescents aged 12.02 ± 0.41 years were analyzed. The quantitative method was validated for linearity, limit of detection, limit of quantification, recovery, intra-and inter-day accuracy and precision, as well as postpreparative stability according to the criteria established by the Association of Official Agricultural Chemists International. A total of 17 aglycones and 37 phase II MPM were identified and quantified in 601 spot urine samples. Phenolic acids were the most abundant urinary MPM, whereas stilbenes, hydroxytyrosol, and enterodiol were the least abundant. Urinary hydroxycoumarin acids (urolithins) were positively correlated with flavonoid and total (poly)phenol intake. An HPLC-ESI-LTQ-Orbitrap-HRMS method was developed and fully validated to quantify MPM. The new method was performed accurately and is suitable for MPM quantification in large epidemiological studies. Urinary lignans and urolithins are proposed as potential biomarkers of grain and nut intake in an adolescent population.
AB - This study aimed to develop and validate a liquid chromatography/electrospray ionization-linear ion trap quadrupole-Orbitrap-high-resolution mass spectrometry (HPLC/ESI-LTQ-Orbitrap-HRMS) method to identify and quantify urinary microbial phenolic metabolites (MPM), as well as to explore the relationship between MPM and dietary (poly)phenols in Spanish adolescents. A total of 601 spot urine samples of adolescents aged 12.02 ± 0.41 years were analyzed. The quantitative method was validated for linearity, limit of detection, limit of quantification, recovery, intra-and inter-day accuracy and precision, as well as postpreparative stability according to the criteria established by the Association of Official Agricultural Chemists International. A total of 17 aglycones and 37 phase II MPM were identified and quantified in 601 spot urine samples. Phenolic acids were the most abundant urinary MPM, whereas stilbenes, hydroxytyrosol, and enterodiol were the least abundant. Urinary hydroxycoumarin acids (urolithins) were positively correlated with flavonoid and total (poly)phenol intake. An HPLC-ESI-LTQ-Orbitrap-HRMS method was developed and fully validated to quantify MPM. The new method was performed accurately and is suitable for MPM quantification in large epidemiological studies. Urinary lignans and urolithins are proposed as potential biomarkers of grain and nut intake in an adolescent population.
KW - biomarker
KW - dietary antioxidants
KW - microbiota
KW - phytochemical
KW - polyphenol
UR - http://www.scopus.com/inward/record.url?scp=85131895520&partnerID=8YFLogxK
U2 - 10.3390/antiox11061167
DO - 10.3390/antiox11061167
M3 - Article
AN - SCOPUS:85131895520
SN - 2076-3921
VL - 11
JO - Antioxidants
JF - Antioxidants
IS - 6
M1 - 1167
ER -