Identification and comprehensive evaluation of a novel biocontrol agent Bacillus atrophaeus JZB120050

Mi Ni; Qiong Wu; Junli Wang; Wei C. Liu; Jian H. Ren; Dian P. Zhang; Juan Zhao; D. E.W. Liu; Ying H. Rao; Cai G. Lu

doi:10.1080/03601234.2018.1505072

Identification and comprehensive evaluation of a novel biocontrol agent Bacillus atrophaeus JZB120050

Mi Ni, Qiong Wu, Junli Wang, Wei C. Liu, Jian H. Ren, Dian P. Zhang, Juan Zhao, D. E.W. Liu, Ying H. Rao, Cai G. Lu

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Bacillus spp. have long been used as biocontrol agents because of their efficient broad-spectrum antimicrobial activity. We identified a novel strain of Bacillus atrophaeus, named JZB120050, from soil. B. atrophaeus JZB120050 had a strong inhibitory effect against Botrytis cinerea and many other phytopathogens. Gas chromatography-mass spectrometry showed that B. atrophaeus JZB120050 produced many secondary metabolites, such as alkanes, alkenes and acids; some of which were related to pathogen inhibition. Enzyme activity analysis showed that B. atrophaeus JZB120050 secreted cell-wall-degrading enzymes, including chitinase, glucanase and protease, which degraded fungal cell walls. Both the novel glucanase gene bglu and chitinase gene chit1 were cloned and heterologously expressed in Escherichia coli and the products showed strong enzyme activity. In addition, B. atrophaeus JZB120050 secreted siderophores and formed a significant biofilm. Future studies should focus on these antimicrobial factors to facilitate widespread application in the field of agricultural biocontrol.

Original language	English
Pages (from-to)	777-785
Number of pages	9
Journal	Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes
Volume	53
Issue number	12
DOIs	https://doi.org/10.1080/03601234.2018.1505072
State	Published - 2 Dec 2018
Externally published	Yes

Keywords

Bacillus atrophaeus
biocontrol
cell-wall-degrading enzymes
heterologous expression
secondary metabolites

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10.1080/03601234.2018.1505072

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Ni, M., Wu, Q., Wang, J., Liu, W. C., Ren, J. H., Zhang, D. P., Zhao, J., Liu, D. E. W., Rao, Y. H., & Lu, C. G. (2018). Identification and comprehensive evaluation of a novel biocontrol agent Bacillus atrophaeus JZB120050. Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes, 53(12), 777-785. https://doi.org/10.1080/03601234.2018.1505072

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abstract = "Bacillus spp. have long been used as biocontrol agents because of their efficient broad-spectrum antimicrobial activity. We identified a novel strain of Bacillus atrophaeus, named JZB120050, from soil. B. atrophaeus JZB120050 had a strong inhibitory effect against Botrytis cinerea and many other phytopathogens. Gas chromatography-mass spectrometry showed that B. atrophaeus JZB120050 produced many secondary metabolites, such as alkanes, alkenes and acids; some of which were related to pathogen inhibition. Enzyme activity analysis showed that B. atrophaeus JZB120050 secreted cell-wall-degrading enzymes, including chitinase, glucanase and protease, which degraded fungal cell walls. Both the novel glucanase gene bglu and chitinase gene chit1 were cloned and heterologously expressed in Escherichia coli and the products showed strong enzyme activity. In addition, B. atrophaeus JZB120050 secreted siderophores and formed a significant biofilm. Future studies should focus on these antimicrobial factors to facilitate widespread application in the field of agricultural biocontrol.",

keywords = "Bacillus atrophaeus, biocontrol, cell-wall-degrading enzymes, heterologous expression, secondary metabolites",

author = "Mi Ni and Qiong Wu and Junli Wang and Liu, {Wei C.} and Ren, {Jian H.} and Zhang, {Dian P.} and Juan Zhao and Liu, {D. E.W.} and Rao, {Ying H.} and Lu, {Cai G.}",

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Ni, M, Wu, Q, Wang, J, Liu, WC, Ren, JH, Zhang, DP, Zhao, J, Liu, DEW, Rao, YH & Lu, CG 2018, 'Identification and comprehensive evaluation of a novel biocontrol agent Bacillus atrophaeus JZB120050', Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes, vol. 53, no. 12, pp. 777-785. https://doi.org/10.1080/03601234.2018.1505072

TY - JOUR

T1 - Identification and comprehensive evaluation of a novel biocontrol agent Bacillus atrophaeus JZB120050

AU - Ni, Mi

AU - Wu, Qiong

AU - Wang, Junli

AU - Liu, Wei C.

AU - Ren, Jian H.

AU - Zhang, Dian P.

AU - Zhao, Juan

AU - Liu, D. E.W.

AU - Rao, Ying H.

AU - Lu, Cai G.

PY - 2018/12/2

Y1 - 2018/12/2

N2 - Bacillus spp. have long been used as biocontrol agents because of their efficient broad-spectrum antimicrobial activity. We identified a novel strain of Bacillus atrophaeus, named JZB120050, from soil. B. atrophaeus JZB120050 had a strong inhibitory effect against Botrytis cinerea and many other phytopathogens. Gas chromatography-mass spectrometry showed that B. atrophaeus JZB120050 produced many secondary metabolites, such as alkanes, alkenes and acids; some of which were related to pathogen inhibition. Enzyme activity analysis showed that B. atrophaeus JZB120050 secreted cell-wall-degrading enzymes, including chitinase, glucanase and protease, which degraded fungal cell walls. Both the novel glucanase gene bglu and chitinase gene chit1 were cloned and heterologously expressed in Escherichia coli and the products showed strong enzyme activity. In addition, B. atrophaeus JZB120050 secreted siderophores and formed a significant biofilm. Future studies should focus on these antimicrobial factors to facilitate widespread application in the field of agricultural biocontrol.

AB - Bacillus spp. have long been used as biocontrol agents because of their efficient broad-spectrum antimicrobial activity. We identified a novel strain of Bacillus atrophaeus, named JZB120050, from soil. B. atrophaeus JZB120050 had a strong inhibitory effect against Botrytis cinerea and many other phytopathogens. Gas chromatography-mass spectrometry showed that B. atrophaeus JZB120050 produced many secondary metabolites, such as alkanes, alkenes and acids; some of which were related to pathogen inhibition. Enzyme activity analysis showed that B. atrophaeus JZB120050 secreted cell-wall-degrading enzymes, including chitinase, glucanase and protease, which degraded fungal cell walls. Both the novel glucanase gene bglu and chitinase gene chit1 were cloned and heterologously expressed in Escherichia coli and the products showed strong enzyme activity. In addition, B. atrophaeus JZB120050 secreted siderophores and formed a significant biofilm. Future studies should focus on these antimicrobial factors to facilitate widespread application in the field of agricultural biocontrol.

KW - Bacillus atrophaeus

KW - biocontrol

KW - cell-wall-degrading enzymes

KW - heterologous expression

KW - secondary metabolites

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VL - 53

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EP - 785

JO - Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes

JF - Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes

IS - 12

ER -

Identification and comprehensive evaluation of a novel biocontrol agent Bacillus atrophaeus JZB120050

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