Identification and characterization of protein complexes from total brain and synaptoneurosomes: Heterogeneity of molecular complexes in distinct subcellular domains

Silvia De Rubeis, Claudia Bagni

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Neurons are highly polarized cells characterized by subcellular microdomains: the synapses. These compartments are specialized structures and are, for certain cellular pathways, independent from the cell body. To achieve such a functional specificity, including local mRNA translation, different molecular complexes are transported along the dendrites and locally regulated. Characterization of such a molecular diversity may help to elucidate neuronal functions as well as detect differences in neuronal dysfunctions. Here, we describe a method to specifically dissect a molecular complex according to the neuronal subcellular compartment. Specifically, the complexes are isolated by immunoprecipitation of the protein of interest from brain lysates or from purified synapses (synaptoneurosomes) and identified by mass spectrometry analysis.

Original languageEnglish
Title of host publicationNeuroproteomics
EditorsKa Wan Li
Pages69-79
Number of pages11
DOIs
StatePublished - 2011

Publication series

NameNeuromethods
Volume57
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • Immunoprecipitation
  • Neurites
  • Synaptic proteomic
  • Synaptoneurosomes
  • mRNP transport

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