Identification and Characterization of p300-Mediated Lysine Residues in Cardiac SERCA2a

Przemek A. Gorski, Ahyoung Lee, Philyoung Lee, Jae Gyun Oh, Peter Vangheluwe, Kiyotake Ishikawa, Roger Hajjar, Changwon Kho

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Impaired calcium uptake resulting from reduced expression and activity of the cardiac sarco-endoplasmic reticulum Ca2+ ATPase (SERCA2a) is a hallmark of heart failure (HF). Recently, new mechanisms of SERCA2a regulation, including post-translational modifications (PTMs), have emerged. Our latest analysis of SERCA2a PTMs has identified lysine acetylation as another PTM which might play a significant role in regulating SERCA2a activity. SERCA2a is acetylated, and that acetylation is more prominent in failing human hearts. In this study, we confirmed that p300 interacts with and acetylates SERCA2a in cardiac tissues. Several lysine residues in SERCA2a modulated by p300 were identified using in vitro acetylation assay. Analysis of in vitro acetylated SERCA2a revealed several lysine residues in SERCA2a susceptible to acetylation by p300. Among them, SERCA2a Lys514 (K514) was confirmed to be essential for SERCA2a activity and stability using an acetylated mimicking mutant. Finally, the reintroduction of an acetyl-mimicking mutant of SERCA2a (K514Q) into SERCA2 knockout cardiomyocytes resulted in deteriorated cardiomyocyte function. Taken together, our data demonstrated that p300-mediated acetylation of SERCA2a is a critical PTM that decreases the pump’s function and contributes to cardiac impairment in HF. SERCA2a acetylation can be targeted for therapeutic aims for the treatment of HF.

Original languageEnglish
Article number3502
JournalInternational Journal of Molecular Sciences
Volume24
Issue number4
DOIs
StatePublished - Feb 2023

Keywords

  • acetyltransferase
  • calcium ATPase
  • cardiac muscle
  • post-translational modifications

Fingerprint

Dive into the research topics of 'Identification and Characterization of p300-Mediated Lysine Residues in Cardiac SERCA2a'. Together they form a unique fingerprint.

Cite this