Identification and Characterization of Novel Antibody Epitopes on the N2 Neuraminidase

Ericka Kirkpatrick Roubidoux, Meagan McMahon, Juan Manuel Carreño, Christina Capuano, Kaijun Jiang, Viviana Simon, Harm van Bakel, Patrick Wilson, Florian Krammer

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


The influenza virus neuraminidase (NA) is becoming a focus for novel vaccine designs. However, the epitopes of human anti-NA antibodies have been poorly defined. Using a panel of 10 anti-N2 monoclonal antibodies (MAbs) that bind the H3N2 virus A/Switzerland/9715293/2013, we generated five escape mutant viruses. These viruses contained mutations K199E/T, E258K, A272D, and S331N. We found that mutations at K199 and E258 had the largest impact on MAb binding, NA inhibition and neutralization activity. In addition, a natural isolate from the 2017- 2018 season was found to contain the E258K mutation and was resistant to numerous antibodies tested. The mutation S331N, was identified in virus passaged in the presence of antibody; however, it had little impact on MAb activity and greatly decreased viral fitness. This information aids in identifying novel human MAb epitopes on the N2 and helps with the detection of antigenically drifted NAs.

Original languageEnglish
Article numbere00958-20
Pages (from-to)1-11
Number of pages11
Issue number1
StatePublished - Jan 2021


  • N2
  • epitopes
  • influenza
  • mAb
  • neuraminidase


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