Abstract
The expression of transforming growth factor β (TGF β) in proximal tubule cells from rabbit kidney cortex after urinephrectomy (UNX) was investigated. Cell protein and [3H]leucine incorporation in these cells were significantly increased, while cell number was decreased, at two weeks following UNX. At this time period after UNX, we found that proximal tubule cells showed a dramatic increase of cytoplasmic immunostaining with a pan-specific anti-TGF β antibody. This was accompanied by a 3-fold increase in TGF β1 mRNA expression in these cells. Furthermore, proximal tubule cells from two-week uninephrectomized rabbits secrete about 2-fold higher TGF β bioactivity to the cell conditioned medium, compared to cells from sham-operated animals. Addition of anti-TGF β,β2,β3 neutralizing antibody increased the growth of the former cells, and it abolished cell hypertrophy. These results indicate that hypertrophy of proximal tubule cells during compensatory renal growth is associated with overexpression of TGF β.
Original language | English |
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Pages (from-to) | 1773-1782 |
Number of pages | 10 |
Journal | Life Sciences |
Volume | 59 |
Issue number | 21 |
DOIs | |
State | Published - 18 Oct 1996 |
Externally published | Yes |
Keywords
- proximal tubule cells
- renal hypertrophy
- transforming growth factor β
- uninephrectomy