Hypertrophy of rabbit proximal tubule cells is associated with overexpression of TGF β

Carlos Peñaranda, Adolfo García-Ocaña, José Luis Sarasa, Pedro Esbrit

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The expression of transforming growth factor β (TGF β) in proximal tubule cells from rabbit kidney cortex after urinephrectomy (UNX) was investigated. Cell protein and [3H]leucine incorporation in these cells were significantly increased, while cell number was decreased, at two weeks following UNX. At this time period after UNX, we found that proximal tubule cells showed a dramatic increase of cytoplasmic immunostaining with a pan-specific anti-TGF β antibody. This was accompanied by a 3-fold increase in TGF β1 mRNA expression in these cells. Furthermore, proximal tubule cells from two-week uninephrectomized rabbits secrete about 2-fold higher TGF β bioactivity to the cell conditioned medium, compared to cells from sham-operated animals. Addition of anti-TGF β,β23 neutralizing antibody increased the growth of the former cells, and it abolished cell hypertrophy. These results indicate that hypertrophy of proximal tubule cells during compensatory renal growth is associated with overexpression of TGF β.

Original languageEnglish
Pages (from-to)1773-1782
Number of pages10
JournalLife Sciences
Volume59
Issue number21
DOIs
StatePublished - 18 Oct 1996
Externally publishedYes

Keywords

  • proximal tubule cells
  • renal hypertrophy
  • transforming growth factor β
  • uninephrectomy

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