Hydrodynamic shear-based purification of cancer cells with enhanced tumorigenic potential

Efraín A. Cermeño, Meghan J. O’Melia, Woojin M. Han, Austin Veith, Graham Barber, Emina H. Huang, Susan N. Thomas, Andrés J. García

Research output: Contribution to journalArticlepeer-review

Abstract

Tumor-initiating cells (TICs), a subpopulation of cancerous cells with high tumorigenic potential and stem-cell-like properties, drive tumor progression and are resistant to conventional therapies. Identification and isolation of TICs are limited by their low frequency and lack of robust markers. Here, we characterize the heterogeneous adhesive properties of a panel of human and murine cancer cells and demonstrate differences in adhesion strength among cells, which exhibit TIC properties and those that do not. These differences in adhesion strength were exploited to rapidly (∼10 min) and efficiently isolate cancerous cells with increased tumorigenic potential in a label-free manner by use of a microfluidic technology. Isolated murine and human cancer cells gave rise to larger tumors with increased growth rate and higher frequency in both immunocompetent and immunocompromised mice, respectively. This rapid and label-free TIC isolation technology has the potential to be a valuable tool for facilitating research into TIC biology and the development of more efficient diagnostics and cancer therapies.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalIntegrative Biology (United Kingdom)
Volume12
Issue number1
DOIs
StatePublished - 1 Jan 2020
Externally publishedYes

Keywords

  • cancer
  • cell adhesion
  • hydrodynamic
  • microfluidic
  • tumor-initiating cells

Fingerprint

Dive into the research topics of 'Hydrodynamic shear-based purification of cancer cells with enhanced tumorigenic potential'. Together they form a unique fingerprint.

Cite this