Hyaluronan- and heparin-reduced silver nanoparticles with antimicrobial properties

Melissa M. Kemp, Ashavani Kumar, Dylan Clement, Pulickel Ajayan, Shaker Mousa, Robert J. Linhardt

Research output: Contribution to journalArticlepeer-review

69 Scopus citations


Aims: Silver nanoparticles exhibit unique antibacterial properties that make these ideal candidates for biological and medical applications. We utilized a clean method involving a single synthetic step to prepare silver nanoparticles that exhibit antimicrobial activity. Materials & methods: These nanoparticles were prepared by reducing silver nitrate with diaminopyridinylated heparin (DAPHP) and hyaluronan (HA) polysaccharides and tested for their efficacy in inhibiting microbial growth. Results & discussion: The resulting silver nanoparticles exhibit potent antimicrobial activity against Staphylococcus aureus and modest activity against Escherichia coli. Silver-HA showed greater antimicrobial activity than silver-DAPHP, while silver-glucose nanoparticles exhibited very weak antimicrobial activity. Neither HA nor DAPHP showed activity against S. aureus or E. coli. Conclusion: These results suggest that DAPHP and HA silver nanoparticles have potential in antimicrobial therapeutic applications.

Original languageEnglish
Pages (from-to)421-429
Number of pages9
Issue number4
StatePublished - 2009
Externally publishedYes


  • Antimicrobial
  • Escherichia coli
  • Silver heparin nanoparticles
  • Silver hyaluronan nanoparticles
  • Silver nanoparticles
  • Staphylococcus aureus


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