Human tumor and rodent-human hybrid cells with an increased number of active human NORs

A human fibrosarcoma line, HT1080-6TG, with a near diploid number of chromosomes. has an average of 7.3 chromosomes with an Ag-stained nucleolus organizer region (NOR). Cells of this line with an increased number of chromosomes have an increased number of Ag-stained NORs. This cell line has been used as (lie human parent in constructing mouse-human and rat-human hybrids that segregate rodent chromosomes. The hybrid cell lines, which have 100 or more chromosomes per cell, show a proportionate increase in the number of Ag-stained NORs (means, 11.4-16.8). The frequency of association of acrocentric chromosomes increases in a similar fashion. There is no evidence of inactivation of human NORs in these cells.