Human trabecular meshwork cells in culture: Morphology and extracellular matrix components

M. R. Hernandez, B. I. Weinstein, J. Schwartz, R. Ritch, G. G. Gordon, A. L. Southren

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

This study demonstrates the presence of laminin and collagen type IV in the extracellular space of human trabecular meshwork (HTM) cells in culture and its absence in cultures of fibroblasts from sclera adjacent to the outflow pathway. These basement membrane components can be detected by standard immunohistochemical techniques. Positive staining for these macromolecules is found only after the HTM cells have reached confluence. The presence of laminin and human collagen type IV in the early passages can serve as additional criteria for identification of HTM cells in culture. These cells provide a useful experimental system for studying the effects of drugs and other factors on the synthesis of components of the extracellular matrix.

Original languageEnglish
Pages (from-to)1655-1660
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume28
Issue number10
StatePublished - 1987
Externally publishedYes

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