TY - JOUR
T1 - Human T Cell Growth Factor (TCGF) produced by repeated stimulation of non-adherent human lymphocytes
AU - Yintang Wu, Wu
AU - Ernberg, Ingemar
AU - Masucci, Maria G.
AU - Johnson, Donald
AU - Klein, Eva
AU - Klein, George
N1 - Funding Information:
This study was supported in part by Contract No. 333 16 from the Division of Cancer Cause and Prevention within the National Institute of Health, the Swedish Cancer Society. Dr. Wu is the recipient of fellowship from the Ministry of Education, People's Republic of China. Dr. Masucci is a recipient of a fellowship from the Foundation Blancefort Boncompagni-Ludovisi (born Bildt), Stockholm, Sweden.
PY - 1982/5/28
Y1 - 1982/5/28
N2 - T cell growth factor (TCGF) has become a valuable means of maintaining T lymphocytes in long-term culture and of studying T cell function. Numerous problems have been met in the production of TCGF of consistently good quality and in the maintenance of human T cell lines over long periods. We have investigated optimal conditions for TCGF production, and simplified assay systems for TCGF activity. The best TCGF production was obtained by short-term treatment with high concentrations of phytohemagglutinin (PHA). The TCGF producing lymphocytes could be re-used for TCGF production up to 1 month after the first treatment course. Human cultured T cell lines, fresh lymphocytes, short-term PHA stimulated lymphocytes and cultured marmoset T lymphocyte lines were all used for assay of TCGF. We recommend PHA stimulation of human lymphocytes for this assay on a routine basis, comparing results with a standard TCGF batch and calculating a growth index. Adherent cells impair TCGF production. Optimal TCGF production was seen when lymphocyte preparations without adherent cells from different donors were used.
AB - T cell growth factor (TCGF) has become a valuable means of maintaining T lymphocytes in long-term culture and of studying T cell function. Numerous problems have been met in the production of TCGF of consistently good quality and in the maintenance of human T cell lines over long periods. We have investigated optimal conditions for TCGF production, and simplified assay systems for TCGF activity. The best TCGF production was obtained by short-term treatment with high concentrations of phytohemagglutinin (PHA). The TCGF producing lymphocytes could be re-used for TCGF production up to 1 month after the first treatment course. Human cultured T cell lines, fresh lymphocytes, short-term PHA stimulated lymphocytes and cultured marmoset T lymphocyte lines were all used for assay of TCGF. We recommend PHA stimulation of human lymphocytes for this assay on a routine basis, comparing results with a standard TCGF batch and calculating a growth index. Adherent cells impair TCGF production. Optimal TCGF production was seen when lymphocyte preparations without adherent cells from different donors were used.
KW - PHA stimulation of TCGF
KW - human T cell growth factor
KW - production of TCGF
UR - http://www.scopus.com/inward/record.url?scp=0020031491&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(82)90379-9
DO - 10.1016/0022-1759(82)90379-9
M3 - Article
C2 - 6809829
AN - SCOPUS:0020031491
SN - 0022-1759
VL - 51
SP - 35
EP - 44
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1
ER -