Human monocytes in culture synthesize and secrete lipoprotein lipase

Patsy Wang-Iverson; Anne Ungar; Jemiliya Bliumis; Phillip R. Bukberg; Joyce C. Gibson; W. Virgil Brown

doi:10.1016/0006-291X(82)91337-7

Human monocytes in culture synthesize and secrete lipoprotein lipase

Patsy Wang-Iverson, Anne Ungar, Jemiliya Bliumis, Phillip R. Bukberg, Joyce C. Gibson, W. Virgil Brown

Icahn School of Medicine at Mount Sinai

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Within the first day in culture, human monocytes begin to synthesize and secrete a triglyceride lipase. The designation of this activity as lipoprotein lipase is based upon: 1) a requirement of serum or apolipoprotein C-II for full activity; 2) inhibition by 1M NaCl or apolipoprotein C-III₂; 3) a pH optimum of 8; and 4) binding to endothelial cells that is releasable by heparin. The enzyme also exhibits immunological cross reactivity with antibody to purified bovine milk lipoprotein lipase as does human postheparin plasma lipoprotein lipase. Lymphocytes and polymorphonuclear leukocytes do not appear to contain this enzyme.

Original language	English
Pages (from-to)	923-928
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	104
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(82)91337-7
State	Published - 11 Feb 1982

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title = "Human monocytes in culture synthesize and secrete lipoprotein lipase",

abstract = "Within the first day in culture, human monocytes begin to synthesize and secrete a triglyceride lipase. The designation of this activity as lipoprotein lipase is based upon: 1) a requirement of serum or apolipoprotein C-II for full activity; 2) inhibition by 1M NaCl or apolipoprotein C-III2; 3) a pH optimum of 8; and 4) binding to endothelial cells that is releasable by heparin. The enzyme also exhibits immunological cross reactivity with antibody to purified bovine milk lipoprotein lipase as does human postheparin plasma lipoprotein lipase. Lymphocytes and polymorphonuclear leukocytes do not appear to contain this enzyme.",

author = "Patsy Wang-Iverson and Anne Ungar and Jemiliya Bliumis and Bukberg, {Phillip R.} and Gibson, {Joyce C.} and Brown, {W. Virgil}",

note = "Funding Information: This work was supported by NIH grants HL 27316 and HL 25752. P.R.B. by Training Grant 2F32 HL 06044.",

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T1 - Human monocytes in culture synthesize and secrete lipoprotein lipase

AU - Wang-Iverson, Patsy

AU - Ungar, Anne

AU - Bliumis, Jemiliya

AU - Bukberg, Phillip R.

AU - Gibson, Joyce C.

AU - Brown, W. Virgil

N1 - Funding Information: This work was supported by NIH grants HL 27316 and HL 25752. P.R.B. by Training Grant 2F32 HL 06044.

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Y1 - 1982/2/11

N2 - Within the first day in culture, human monocytes begin to synthesize and secrete a triglyceride lipase. The designation of this activity as lipoprotein lipase is based upon: 1) a requirement of serum or apolipoprotein C-II for full activity; 2) inhibition by 1M NaCl or apolipoprotein C-III2; 3) a pH optimum of 8; and 4) binding to endothelial cells that is releasable by heparin. The enzyme also exhibits immunological cross reactivity with antibody to purified bovine milk lipoprotein lipase as does human postheparin plasma lipoprotein lipase. Lymphocytes and polymorphonuclear leukocytes do not appear to contain this enzyme.

AB - Within the first day in culture, human monocytes begin to synthesize and secrete a triglyceride lipase. The designation of this activity as lipoprotein lipase is based upon: 1) a requirement of serum or apolipoprotein C-II for full activity; 2) inhibition by 1M NaCl or apolipoprotein C-III2; 3) a pH optimum of 8; and 4) binding to endothelial cells that is releasable by heparin. The enzyme also exhibits immunological cross reactivity with antibody to purified bovine milk lipoprotein lipase as does human postheparin plasma lipoprotein lipase. Lymphocytes and polymorphonuclear leukocytes do not appear to contain this enzyme.

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Human monocytes in culture synthesize and secrete lipoprotein lipase

Abstract

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