Human Innate Lymphoid Cell Subsets Possess Tissue-Type Based Heterogeneity in Phenotype and Frequency

Yannick Simoni; Michael Fehlings; Henrik N. Kløverpris; Naomi McGovern; Si Lin Koo; Chiew Yee Loh; Shawn Lim; Ayako Kurioka; Joannah R. Fergusson; Choong Leong Tang; Ming Hian Kam; Koh Dennis; Tony Kiat Hon Lim; Alexander Chung Yaw Fui; Chan Weng Hoong; Jerry Kok Yen Chan; Maria Curotto de Lafaille; Sriram Narayanan; Sonia Baig; Muhammad Shabeer; Sue Anne Ee Shiow Toh; Henry Kun Kiaang Tan; Rosslyn Anicete; Eng Huat Tan; Angela Takano; Paul Klenerman; Alasdair Leslie; Daniel S.W. Tan; Iain Beehuat Tan; Florent Ginhoux; Evan W. Newell

doi:10.1016/j.immuni.2016.11.005

Human Innate Lymphoid Cell Subsets Possess Tissue-Type Based Heterogeneity in Phenotype and Frequency

Yannick Simoni, Michael Fehlings, Henrik N. Kløverpris, Naomi McGovern, Si Lin Koo, Chiew Yee Loh, Shawn Lim, Ayako Kurioka, Joannah R. Fergusson, Choong Leong Tang, Ming Hian Kam, Koh Dennis, Tony Kiat Hon Lim, Alexander Chung Yaw Fui, Chan Weng Hoong, Jerry Kok Yen Chan, Maria Curotto de Lafaille, Sriram Narayanan, Sonia Baig, Muhammad ShabeerSue Anne Ee Shiow Toh, Henry Kun Kiaang Tan, Rosslyn Anicete, Eng Huat Tan, Angela Takano, Paul Klenerman, Alasdair Leslie, Daniel S.W. Tan, Iain Beehuat Tan, Florent Ginhoux, Evan W. Newell

Research output: Contribution to journal › Article › peer-review

348 Scopus citations

Abstract

Animal models have highlighted the importance of innate lymphoid cells (ILCs) in multiple immune responses. However, technical limitations have hampered adequate characterization of ILCs in humans. Here, we used mass cytometry including a broad range of surface markers and transcription factors to accurately identify and profile ILCs across healthy and inflamed tissue types. High dimensional analysis allowed for clear phenotypic delineation of ILC2 and ILC3 subsets. We were not able to detect ILC1 cells in any of the tissues assessed, however, we identified intra-epithelial (ie)ILC1-like cells that represent a broader category of NK cells in mucosal and non-mucosal pathological tissues. In addition, we have revealed the expression of phenotypic molecules that have not been previously described for ILCs. Our analysis shows that human ILCs are highly heterogeneous cell types between individuals and tissues. It also provides a global, comprehensive, and detailed description of ILC heterogeneity in humans across patients and tissues.

Original language	English
Pages (from-to)	148-161
Number of pages	14
Journal	Immunity
Volume	46
Issue number	1
DOIs	https://doi.org/10.1016/j.immuni.2016.11.005
State	Published - 17 Jan 2017
Externally published	Yes

Keywords

CyTOF
ILC
ILC1
ILC2
ILC3
human
ieILC1

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10.1016/j.immuni.2016.11.005

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Simoni, Y., Fehlings, M., Kløverpris, H. N., McGovern, N., Koo, S. L., Loh, C. Y., Lim, S., Kurioka, A., Fergusson, J. R., Tang, C. L., Kam, M. H., Dennis, K., Lim, T. K. H., Fui, A. C. Y., Hoong, C. W., Chan, J. K. Y., Curotto de Lafaille, M., Narayanan, S., Baig, S., ... Newell, E. W. (2017). Human Innate Lymphoid Cell Subsets Possess Tissue-Type Based Heterogeneity in Phenotype and Frequency. Immunity, 46(1), 148-161. https://doi.org/10.1016/j.immuni.2016.11.005

@article{f051e9317c1045f5a4945e15c5e94912,

title = "Human Innate Lymphoid Cell Subsets Possess Tissue-Type Based Heterogeneity in Phenotype and Frequency",

abstract = "Animal models have highlighted the importance of innate lymphoid cells (ILCs) in multiple immune responses. However, technical limitations have hampered adequate characterization of ILCs in humans. Here, we used mass cytometry including a broad range of surface markers and transcription factors to accurately identify and profile ILCs across healthy and inflamed tissue types. High dimensional analysis allowed for clear phenotypic delineation of ILC2 and ILC3 subsets. We were not able to detect ILC1 cells in any of the tissues assessed, however, we identified intra-epithelial (ie)ILC1-like cells that represent a broader category of NK cells in mucosal and non-mucosal pathological tissues. In addition, we have revealed the expression of phenotypic molecules that have not been previously described for ILCs. Our analysis shows that human ILCs are highly heterogeneous cell types between individuals and tissues. It also provides a global, comprehensive, and detailed description of ILC heterogeneity in humans across patients and tissues.",

keywords = "CyTOF, ILC, ILC1, ILC2, ILC3, human, ieILC1",

author = "Yannick Simoni and Michael Fehlings and Kl{\o}verpris, {Henrik N.} and Naomi McGovern and Koo, {Si Lin} and Loh, {Chiew Yee} and Shawn Lim and Ayako Kurioka and Fergusson, {Joannah R.} and Tang, {Choong Leong} and Kam, {Ming Hian} and Koh Dennis and Lim, {Tony Kiat Hon} and Fui, {Alexander Chung Yaw} and Hoong, {Chan Weng} and Chan, {Jerry Kok Yen} and {Curotto de Lafaille}, Maria and Sriram Narayanan and Sonia Baig and Muhammad Shabeer and Toh, {Sue Anne Ee Shiow} and Tan, {Henry Kun Kiaang} and Rosslyn Anicete and Tan, {Eng Huat} and Angela Takano and Paul Klenerman and Alasdair Leslie and Tan, {Daniel S.W.} and Tan, {Iain Beehuat} and Florent Ginhoux and Newell, {Evan W.}",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",

year = "2017",

month = jan,

day = "17",

doi = "10.1016/j.immuni.2016.11.005",

language = "English",

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Simoni, Y, Fehlings, M, Kløverpris, HN, McGovern, N, Koo, SL, Loh, CY, Lim, S, Kurioka, A, Fergusson, JR, Tang, CL, Kam, MH, Dennis, K, Lim, TKH, Fui, ACY, Hoong, CW, Chan, JKY, Curotto de Lafaille, M, Narayanan, S, Baig, S, Shabeer, M, Toh, SAES, Tan, HKK, Anicete, R, Tan, EH, Takano, A, Klenerman, P, Leslie, A, Tan, DSW, Tan, IB, Ginhoux, F & Newell, EW 2017, 'Human Innate Lymphoid Cell Subsets Possess Tissue-Type Based Heterogeneity in Phenotype and Frequency', Immunity, vol. 46, no. 1, pp. 148-161. https://doi.org/10.1016/j.immuni.2016.11.005

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AU - Simoni, Yannick

AU - Fehlings, Michael

AU - Kløverpris, Henrik N.

AU - McGovern, Naomi

AU - Koo, Si Lin

AU - Loh, Chiew Yee

AU - Lim, Shawn

AU - Kurioka, Ayako

AU - Fergusson, Joannah R.

AU - Tang, Choong Leong

AU - Kam, Ming Hian

AU - Dennis, Koh

AU - Lim, Tony Kiat Hon

AU - Fui, Alexander Chung Yaw

AU - Hoong, Chan Weng

AU - Chan, Jerry Kok Yen

AU - Curotto de Lafaille, Maria

AU - Narayanan, Sriram

AU - Baig, Sonia

AU - Shabeer, Muhammad

AU - Toh, Sue Anne Ee Shiow

AU - Tan, Henry Kun Kiaang

AU - Anicete, Rosslyn

AU - Tan, Eng Huat

AU - Takano, Angela

AU - Klenerman, Paul

AU - Leslie, Alasdair

AU - Tan, Daniel S.W.

AU - Tan, Iain Beehuat

AU - Ginhoux, Florent

AU - Newell, Evan W.

PY - 2017/1/17

Y1 - 2017/1/17

N2 - Animal models have highlighted the importance of innate lymphoid cells (ILCs) in multiple immune responses. However, technical limitations have hampered adequate characterization of ILCs in humans. Here, we used mass cytometry including a broad range of surface markers and transcription factors to accurately identify and profile ILCs across healthy and inflamed tissue types. High dimensional analysis allowed for clear phenotypic delineation of ILC2 and ILC3 subsets. We were not able to detect ILC1 cells in any of the tissues assessed, however, we identified intra-epithelial (ie)ILC1-like cells that represent a broader category of NK cells in mucosal and non-mucosal pathological tissues. In addition, we have revealed the expression of phenotypic molecules that have not been previously described for ILCs. Our analysis shows that human ILCs are highly heterogeneous cell types between individuals and tissues. It also provides a global, comprehensive, and detailed description of ILC heterogeneity in humans across patients and tissues.

AB - Animal models have highlighted the importance of innate lymphoid cells (ILCs) in multiple immune responses. However, technical limitations have hampered adequate characterization of ILCs in humans. Here, we used mass cytometry including a broad range of surface markers and transcription factors to accurately identify and profile ILCs across healthy and inflamed tissue types. High dimensional analysis allowed for clear phenotypic delineation of ILC2 and ILC3 subsets. We were not able to detect ILC1 cells in any of the tissues assessed, however, we identified intra-epithelial (ie)ILC1-like cells that represent a broader category of NK cells in mucosal and non-mucosal pathological tissues. In addition, we have revealed the expression of phenotypic molecules that have not been previously described for ILCs. Our analysis shows that human ILCs are highly heterogeneous cell types between individuals and tissues. It also provides a global, comprehensive, and detailed description of ILC heterogeneity in humans across patients and tissues.

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KW - ILC

KW - ILC1

KW - ILC2

KW - ILC3

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KW - ieILC1

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DO - 10.1016/j.immuni.2016.11.005

M3 - Article

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AN - SCOPUS:85008239935

SN - 1074-7613

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JO - Immunity

JF - Immunity

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ER -

Human Innate Lymphoid Cell Subsets Possess Tissue-Type Based Heterogeneity in Phenotype and Frequency

Abstract

Keywords

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