TY - JOUR
T1 - Human Immunodeficiency Virus Type 1 Infection Requires Reverse Transcription of Nascent Viral RNA
AU - Potash, Mary Jane
AU - li, Gongrong
AU - Shahabuddin, Muhammad
AU - Pellegrino, Michael G.
AU - Volsky, David J.
PY - 1993/10
Y1 - 1993/10
N2 - We have previously shown that during human immunodeficiency virus type 1 (HIV-1) infection in vitro continued reverse transcription is required for stable HIV-1 production, but entry by progeny virus is not. To determine the source of the viral RNA reverse-transcribed late in infection, we employed inhibitors of HIV-1 transmission, reverse transcription, and proteolysis of the Gag-Pol polyprotein to interrupt HIV-1 infection in vitro. The kinetics of synthesis of viral DNA, RNA, and proteins was examined. During single-cycle infection, inhibition of reverse transcription 24–72 hr after infection delayed production of viral RNA and protein 10 days. Although viral DNA was detected in Southern blots, inhibition of Gag-Pol processing or transient inhibition of reverse transcription blocked its expression. We propose that after initial reverse transcription of input virion RNA is complete, newly synthesized HIV-1 RNA is reverse-transcribed before its export in virions to yield the viral DNA required for stable HIV-1 production.
AB - We have previously shown that during human immunodeficiency virus type 1 (HIV-1) infection in vitro continued reverse transcription is required for stable HIV-1 production, but entry by progeny virus is not. To determine the source of the viral RNA reverse-transcribed late in infection, we employed inhibitors of HIV-1 transmission, reverse transcription, and proteolysis of the Gag-Pol polyprotein to interrupt HIV-1 infection in vitro. The kinetics of synthesis of viral DNA, RNA, and proteins was examined. During single-cycle infection, inhibition of reverse transcription 24–72 hr after infection delayed production of viral RNA and protein 10 days. Although viral DNA was detected in Southern blots, inhibition of Gag-Pol processing or transient inhibition of reverse transcription blocked its expression. We propose that after initial reverse transcription of input virion RNA is complete, newly synthesized HIV-1 RNA is reverse-transcribed before its export in virions to yield the viral DNA required for stable HIV-1 production.
UR - http://www.scopus.com/inward/record.url?scp=0027722309&partnerID=8YFLogxK
U2 - 10.1089/dna.1993.12.685
DO - 10.1089/dna.1993.12.685
M3 - Article
C2 - 7691074
AN - SCOPUS:0027722309
SN - 1044-5498
VL - 12
SP - 685
EP - 693
JO - DNA and Cell Biology
JF - DNA and Cell Biology
IS - 8
ER -