TY - JOUR
T1 - Human Decidual Natural Killer Cells Are a Unique NK Cell Subset with Immunomodulatory Potential
AU - Koopman, Louise A.
AU - Kopcow, Hernan D.
AU - Rybalov, Basya
AU - Boyson, Jonathan E.
AU - Orange, Jordan S.
AU - Schatz, Frederick
AU - Masch, Rachel
AU - Lockwood, Charles J.
AU - Schachter, Asher D.
AU - Park, Peter J.
AU - Strominger, Jack L.
PY - 2003/10/20
Y1 - 2003/10/20
N2 - Natural killer cells constitute 50-90% of lymphocytes in human uterine decidua in early pregnancy. Here, CD56bright uterine decidual NK (dNK) cells were compared with the CD56bright and CD56dim peripheral NK cell subsets by microarray analysis, with verification of results by flow cytometry and RT-PCR. Among the ∼10,000 genes studied, 278 genes showed at least a threefold change with P ≤ 0.001 when comparing the dNK and peripheral NK cell subsets, most displaying increased expression in dNK cells. The largest number of these encoded surface proteins, including the unusual lectinlike receptors NKG2E and Ly-49L, several killer cell Ig-like receptors, the integrin subunits αD, αX, β1, and β5, and multiple tetraspanins (CD9, CD151, CD53, CD63, and TSPAN-5). Additionally, two secreted proteins, galectin-1 and progestagen-associated protein 14, known to have immunomodulatory functions, were selectively expressed in dNK cells.
AB - Natural killer cells constitute 50-90% of lymphocytes in human uterine decidua in early pregnancy. Here, CD56bright uterine decidual NK (dNK) cells were compared with the CD56bright and CD56dim peripheral NK cell subsets by microarray analysis, with verification of results by flow cytometry and RT-PCR. Among the ∼10,000 genes studied, 278 genes showed at least a threefold change with P ≤ 0.001 when comparing the dNK and peripheral NK cell subsets, most displaying increased expression in dNK cells. The largest number of these encoded surface proteins, including the unusual lectinlike receptors NKG2E and Ly-49L, several killer cell Ig-like receptors, the integrin subunits αD, αX, β1, and β5, and multiple tetraspanins (CD9, CD151, CD53, CD63, and TSPAN-5). Additionally, two secreted proteins, galectin-1 and progestagen-associated protein 14, known to have immunomodulatory functions, were selectively expressed in dNK cells.
KW - Gene expression proflling
KW - Lymphocyte subsets
KW - Maternal-fetal relations
KW - Natural killer cells
KW - Pregnancy
UR - http://www.scopus.com/inward/record.url?scp=0142157002&partnerID=8YFLogxK
U2 - 10.1084/jem.20030305
DO - 10.1084/jem.20030305
M3 - Article
C2 - 14568979
AN - SCOPUS:0142157002
SN - 0022-1007
VL - 198
SP - 1201
EP - 1212
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 8
ER -