Human cell lines stably expressing HIV env and tat gene products

Miguel A. Gama Sosa, Rita DeGasperi, Fatemeh Fazely, Ruth M. Ruprecht

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

A DNA fragment containing the tat, rev and env genes of the human immunodeficiency virus type 1 was inserted into the retroviral vector pZIPneoAU3. The resulting plasmid penvAU3 was transfected into HeLa and ψCRIP cells. Resulting recombinant retroviruses were used to infect HeLa and Jurkat cells. Immunoprecipitation analysis of stable transformants showed the expression of HIV env glycoproteins gp160, gp120 and gp41. Transactivation assays with a plasmid containing the gene for chloramphenicol acetyltransferase linked to HIV promoter-enhancer sequences demonstrated the expression of functional tat. These cells constitute virus-free tools for functional and structural studies of native env and tat.

Original languageEnglish
Pages (from-to)305-311
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume161
Issue number1
DOIs
StatePublished - 30 May 1989
Externally publishedYes

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