Human CD46 aberrant splicing in transgenic mice

Lubbertus C.F. Mulder; Mara Rossini; Marirosa Mora

doi:10.1016/S0378-1119(96)00685-3

Human CD46 aberrant splicing in transgenic mice

Lubbertus C.F. Mulder, Mara Rossini, Marirosa Mora

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

RNA analysis of mice transgenic for human CD46 reveals almost undetectable levels of the expected transcript and the accumulation of a 900 nt shorter species. cDNA cloning and sequence analysis of this variant demonstrate an aberrant splicing of the transgene RNA. This aberrant splicing is present at low levels in cells that normally express the gene, suggesting that the transgene is lacking regulatory elements influencing the abundance of the shorter transcript.

Original language	English
Pages (from-to)	83-86
Number of pages	4
Journal	Gene
Volume	186
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1119(96)00685-3
State	Published - 20 Feb 1997
Externally published	Yes

Keywords

Oversplicing
Transgene

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10.1016/S0378-1119(96)00685-3

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title = "Human CD46 aberrant splicing in transgenic mice",

abstract = "RNA analysis of mice transgenic for human CD46 reveals almost undetectable levels of the expected transcript and the accumulation of a 900 nt shorter species. cDNA cloning and sequence analysis of this variant demonstrate an aberrant splicing of the transgene RNA. This aberrant splicing is present at low levels in cells that normally express the gene, suggesting that the transgene is lacking regulatory elements influencing the abundance of the shorter transcript.",

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author = "Mulder, {Lubbertus C.F.} and Mara Rossini and Marirosa Mora",

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AU - Mulder, Lubbertus C.F.

AU - Rossini, Mara

AU - Mora, Marirosa

PY - 1997/2/20

Y1 - 1997/2/20

N2 - RNA analysis of mice transgenic for human CD46 reveals almost undetectable levels of the expected transcript and the accumulation of a 900 nt shorter species. cDNA cloning and sequence analysis of this variant demonstrate an aberrant splicing of the transgene RNA. This aberrant splicing is present at low levels in cells that normally express the gene, suggesting that the transgene is lacking regulatory elements influencing the abundance of the shorter transcript.

AB - RNA analysis of mice transgenic for human CD46 reveals almost undetectable levels of the expected transcript and the accumulation of a 900 nt shorter species. cDNA cloning and sequence analysis of this variant demonstrate an aberrant splicing of the transgene RNA. This aberrant splicing is present at low levels in cells that normally express the gene, suggesting that the transgene is lacking regulatory elements influencing the abundance of the shorter transcript.

KW - Oversplicing

KW - Transgene

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DO - 10.1016/S0378-1119(96)00685-3

M3 - Article

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AN - SCOPUS:0031579436

SN - 0378-1119

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JO - Gene

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Human CD46 aberrant splicing in transgenic mice

Abstract

Keywords

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