Human bone marrow CFU-GM and BFU-E localized by light scatter cell sorting

J. Atzpodien; S. C. Gulati; J. H. Kwon; M. Wachter; J. Fried; B. D. Clarkson

doi:10.1159/000163425

Human bone marrow CFU-GM and BFU-E localized by light scatter cell sorting

J. Atzpodien, S. C. Gulati, J. H. Kwon, M. Wachter, J. Fried, B. D. Clarkson

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3 Scopus citations

Abstract

Flow cytometric separation was performed on the normal human bone marrow (BM) by using the low-angle (0°) or high-angle (90°) light scatter. Four distinct subpopulations of cells can be enriched from normal human BM and these fractions were subsequently evaluated for their morphological properties as well as their clonogenic capacity in various progenitor cell assays. Our results indicate that human erythroid and granulocyte-macrophage progenitor cells can be separated from BM low-density cells by cell sorting, and these cells show similar 0° and 90° light scatter properties to those observed with murine bone marrow studies. Flow cytometric analysis also suggests that the majority of sorted BFU-E and CFU-GM resides in the blast cell subset of human BM mononuclear cells.

Original language	English
Pages (from-to)	265-270
Number of pages	6
Journal	Experimental Cell Biology
Volume	55
Issue number	5
DOIs	https://doi.org/10.1159/000163425
State	Published - 1987
Externally published	Yes

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title = "Human bone marrow CFU-GM and BFU-E localized by light scatter cell sorting",

abstract = "Flow cytometric separation was performed on the normal human bone marrow (BM) by using the low-angle (0°) or high-angle (90°) light scatter. Four distinct subpopulations of cells can be enriched from normal human BM and these fractions were subsequently evaluated for their morphological properties as well as their clonogenic capacity in various progenitor cell assays. Our results indicate that human erythroid and granulocyte-macrophage progenitor cells can be separated from BM low-density cells by cell sorting, and these cells show similar 0° and 90° light scatter properties to those observed with murine bone marrow studies. Flow cytometric analysis also suggests that the majority of sorted BFU-E and CFU-GM resides in the blast cell subset of human BM mononuclear cells.",

author = "J. Atzpodien and Gulati, \{S. C.\} and Kwon, \{J. H.\} and M. Wachter and J. Fried and Clarkson, \{B. D.\}",

year = "1987",

doi = "10.1159/000163425",

language = "English",

volume = "55",

pages = "265--270",

journal = "Experimental Cell Biology",

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TY - JOUR

T1 - Human bone marrow CFU-GM and BFU-E localized by light scatter cell sorting

AU - Atzpodien, J.

AU - Gulati, S. C.

AU - Kwon, J. H.

AU - Wachter, M.

AU - Fried, J.

AU - Clarkson, B. D.

PY - 1987

Y1 - 1987

N2 - Flow cytometric separation was performed on the normal human bone marrow (BM) by using the low-angle (0°) or high-angle (90°) light scatter. Four distinct subpopulations of cells can be enriched from normal human BM and these fractions were subsequently evaluated for their morphological properties as well as their clonogenic capacity in various progenitor cell assays. Our results indicate that human erythroid and granulocyte-macrophage progenitor cells can be separated from BM low-density cells by cell sorting, and these cells show similar 0° and 90° light scatter properties to those observed with murine bone marrow studies. Flow cytometric analysis also suggests that the majority of sorted BFU-E and CFU-GM resides in the blast cell subset of human BM mononuclear cells.

AB - Flow cytometric separation was performed on the normal human bone marrow (BM) by using the low-angle (0°) or high-angle (90°) light scatter. Four distinct subpopulations of cells can be enriched from normal human BM and these fractions were subsequently evaluated for their morphological properties as well as their clonogenic capacity in various progenitor cell assays. Our results indicate that human erythroid and granulocyte-macrophage progenitor cells can be separated from BM low-density cells by cell sorting, and these cells show similar 0° and 90° light scatter properties to those observed with murine bone marrow studies. Flow cytometric analysis also suggests that the majority of sorted BFU-E and CFU-GM resides in the blast cell subset of human BM mononuclear cells.

UR - https://www.scopus.com/pages/publications/0023516771

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DO - 10.1159/000163425

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AN - SCOPUS:0023516771

SN - 0304-3568

VL - 55

SP - 265

EP - 270

JO - Experimental Cell Biology

JF - Experimental Cell Biology

IS - 5

ER -

Human bone marrow CFU-GM and BFU-E localized by light scatter cell sorting

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