Huangqi decoction inhibits hyperglycemia-induced podocyte apoptosis by down-regulated nox4/p53/bax signaling in vitro and in vivo

Zezheng Li, Wenjuan Deng, Aili Cao, Yingjun Zang, Yunman Wang, Hao Wang, Li Wang, Wen Peng

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Background: Podocyte dysfunction is associated with the progression of diabetic nephropathy (DN). Huangqi decoction (HQD), a traditional Chinese medical formula, has been used to improve diabetes-related syndrome in China. The present study was to investigate the protective effect of HQD on podocyte apoptosis and the underlying molecular mechanism. Methods: Podocyte was used to measure the efficacy of HQD on cell apoptosis, activities of NADPH oxidases, ROS generation and mitochondrial membrane potential (MMP), and the activation of Nox4/p53/Bax signaling pathway with HQD treatment were also investigated in vitro. Renal pathological morphology, renal function, podocyte apoptosis and Nox4/p53/Bax signaling pathway were investigated with STZ-induced diabetic mice in vivo. Results: HQD increased the cell proliferation and MMP level, while the ROS production and activities of NADPH oxidases were decreased. Meanwhile, Nox4/p53/Bax signaling was down-regulated. Contrarily, overexpression of Nox4 or p53 significantly abolished those efficacies of HQD. Accordingly, in vivo study showed that the progressive albuminuria, glomerulosclerosis and loss of podocytes were significantly alleviated with HQD treatment in diabetic mice, which paralleled by the marked inhibition of Nox4/p53/Bax signaling. Conclusion: Collectively, we provide further evidence that HQD had a renoprotective effect in preventing podocyte apoptosis, which was mediated at least in part by down-regulation of Nox4/p53/Bax signaling.

Original languageEnglish
Article numberAJTR0085336
Pages (from-to)3195-3212
Number of pages18
JournalAmerican Journal of Translational Research
Volume11
Issue number5
StatePublished - 2019
Externally publishedYes

Keywords

  • Diabetic nephropathy
  • Huangqi decoction
  • Mitochondrial dysfunction
  • Nox4/p53/Bax signaling
  • Oxidative stress
  • Podocytes apoptosis

Fingerprint

Dive into the research topics of 'Huangqi decoction inhibits hyperglycemia-induced podocyte apoptosis by down-regulated nox4/p53/bax signaling in vitro and in vivo'. Together they form a unique fingerprint.

Cite this