Hsa-miR-1269a up-regulation fosters the malignant progression of esophageal squamous cell carcinoma via targeting FAM46C

Yuefeng Ma, Xin Xing, Chuantao Cheng, Ranran Kong, Liangzhang Sun, Feng Zhao, Danjie Zhang, Jianzhong Li

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Esophageal squamous cell carcinoma (ESCC) is a malignancy of the alimentary tract resulting in death worldwide. The role and underlying mechanism of hsa-miR-1269a in the progression of ESCC remain unclear. In this study, hsa-miR-1269a was screened by differential expression analysis in TCGA, and its target gene FAM46C was predicted. qRT-PCR was conducted to assay the expression of hsa-miR-1269a and FAM46C in ESCC cells. The results showed that hsa-miR-1269a was upregulated in ESCC tissues and cell lines. Hsa-miR-1269a overexpression stimulated the proliferation, migration, and invasion capacities of ESCC cells, and FAM46C overexpression inhibited these phenotypes. Dual-luciferase assay verified that hsa-miR-1269a could target FAM46C. Next, qRT-PCR and western blot demonstrated that hsa-miR-1269a overexpression downregulated FAM46C. Rescue experiments revealed that hsa-miR-1269a accelerated the malignant progression of ESCC through FAM46C down-regulation. These results indicate that the interaction between hsa-miR-1269a and FAM46C plays a regulatory role in driving the malignant progression of ESCC cells, thereby providing a novel molecular mechanism for understanding ESCC.

Original languageEnglish
Article number111832
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume827
DOIs
StatePublished - 1 Jul 2023
Externally publishedYes

Keywords

  • Apoptosis
  • Esophageal squamous cell carcinoma
  • FAM46C
  • Hsa-miR-1269a
  • Invasion
  • Migration
  • Proliferation

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