HS-23, a Lonicera japonica extract, reverses sepsis-induced immunosuppression by inhibiting lymphocyte apoptosis

So Jin Kim, Joon Sung Kim, Hyo Sun Choi, Young Mok Kim, Sung Woon Hong, Sung Hum Yeon, Yeon Kim, Sun Mee Lee

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Ethnopharmacological relevance Lonicera japonica Thunberg, a widely used traditional Chinese medicine, possesses antibacterial, antiviral, and antiendotoxin activities. This study investigated the molecular mechanisms of HS-23, the ethanol extract of the dried flower buds of L. japonica, on sepsis-induced immunosuppression. Materials and methods Male ICR mice were intravenously administered HS-23 (10, 20, and 40 mg/kg) immediately (0 h) and 22 h after cecal ligation and puncture (CLP). The spleen was isolated for biochemical assays 24 h after CLP. Results HS-23 improved sepsis-induced mortality. CLP induced a marked decrease in the number of splenocytes, B cells, and natural killer cells, which was attenuated by HS-23. HS-23 also attenuated CLP-induced apoptosis in CD4+ and CD8+ T cells and inhibited both the intrinsic and extrinsic apoptotic pathway in the spleen. HS-23 attenuated the CLP-induced decrease in interleukin (IL)-17 production. CLP significantly decreased splenic production of tumor necrosis factor-α and IL-2, and these effects were attenuated by HS-23. Conclusion Our findings suggest that HS-23 reverses immunosuppression during the late phase of sepsis by inhibiting lymphocyte apoptosis and enhancing Th1 cytokine production. HS-23 warrants further evaluation as a potential therapeutic agent for the treatment of sepsis.

Original languageEnglish
Pages (from-to)231-239
Number of pages9
JournalJournal of Ethnopharmacology
Volume171
DOIs
StatePublished - 22 Jun 2015
Externally publishedYes

Keywords

  • Immunosuppression
  • Lonicera japonica
  • Lymphocyte apoptosis
  • Sepsis
  • Th1/Th2 cytokine

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