How the glycosyltransferase OGT catalyzes amide bond cleavage

John Janetzko, Sunia A. Trauger, Michael B. Lazarus, Suzanne Walker

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


The essential human enzyme O-linked β-N-acetylglucosamine transferase (OGT), known for modulating the functions of nuclear and cytoplasmic proteins through serine and threonine glycosylation, was unexpectedly implicated in the proteolytic maturation of the cell cycle regulator host cell factor-1 (HCF-1). Here we show that HCF-1 cleavage occurs via glycosylation of a glutamate side chain followed by on-enzyme formation of an internal pyroglutamate, which undergoes spontaneous backbone hydrolysis.

Original languageEnglish
Pages (from-to)900-901
Number of pages2
JournalNature Chemical Biology
Issue number11
StatePublished - 1 Nov 2016


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