How Single-Cell Technologies Have Provided New Insights into Atherosclerosis

Natalia Eberhardt, Chiara Giannarelli

Research output: Contribution to journalReview articlepeer-review

3 Scopus citations

Abstract

The development of innovative single-cell technologies has allowed the high-dimensional transcriptomic and proteomic profiling of individual blood and tissue cells. Recent single-cell studies revealed a new cellular heterogeneity of atherosclerotic plaque tissue and allowed a better understanding of distinct immune functional states in the context of atherosclerosis. In this brief review, we describe how single-cell technologies have shed a new light on the cellular composition of atherosclerotic plaques, and their response to diet perturbations or genetic manipulation in mouse models of atherosclerosis. We discuss how single-cell RNA sequencing, cellular indexing of transcriptomes and epitopes by sequencing, transposase-accessible chromatin with high-throughput sequencing, and cytometry by time-of-flight platforms have empowered the identification of discrete immune, endothelial, and smooth muscle cell alterations in atherosclerosis progression and regression. Finally, we review how single-cell approaches have allowed mapping the cellular and molecular composition of human atherosclerotic plaques and the discovery of new immune alterations in plaques from patients with stroke.

Original languageEnglish
Pages (from-to)243-252
Number of pages10
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume42
Issue number3
DOIs
StatePublished - 1 Mar 2022
Externally publishedYes

Keywords

  • atherosclerosis
  • cardiovascular disease
  • cholesterol
  • macrophage
  • proteomics
  • transcriptomics

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