TY - JOUR
T1 - How complementary targets expose the microRNA 3' End for Tailing and Trimming during Target-Directed microRNA Degradation
AU - Pawlica, Paulina
AU - Sheu-Gruttadauria, Jessica
AU - MacRae, Ian J.
AU - Steitz, Joan A.
N1 - Publisher Copyright:
© 2019 Pawlica et al.
PY - 2019
Y1 - 2019
N2 - microRNAs (miRNAs) are crucial for posttranscriptional regulation of messenger RNAs. "Classical"miRNA targets predominantly interact with the miRNA seed sequence located near the miRNA 50 end. Interestingly, certain transcripts that exhibit extensive complementarity to the miRNAs 30 region, instead of being subjected to regulation, induce miRNA decay in a process termed target-directed miRNA degradation (TDMD). Here, we review recent advances in understanding the molecular mechanisms of TDMD. Specifically, we discuss how extensive miRNA complementarity to TDMD-inducing targets results in displacement of the miRNA 30 end from its protective pocket in the Argonaute protein. Unprotected miRNA 30 ends are then available for enzymatic attack by still-unidentified cellular enzymes. Identification of these cellular enzymes and discovery of additional TDMD-inducing transcripts are subjects for future research.
AB - microRNAs (miRNAs) are crucial for posttranscriptional regulation of messenger RNAs. "Classical"miRNA targets predominantly interact with the miRNA seed sequence located near the miRNA 50 end. Interestingly, certain transcripts that exhibit extensive complementarity to the miRNAs 30 region, instead of being subjected to regulation, induce miRNA decay in a process termed target-directed miRNA degradation (TDMD). Here, we review recent advances in understanding the molecular mechanisms of TDMD. Specifically, we discuss how extensive miRNA complementarity to TDMD-inducing targets results in displacement of the miRNA 30 end from its protective pocket in the Argonaute protein. Unprotected miRNA 30 ends are then available for enzymatic attack by still-unidentified cellular enzymes. Identification of these cellular enzymes and discovery of additional TDMD-inducing transcripts are subjects for future research.
UR - http://www.scopus.com/inward/record.url?scp=85088608375&partnerID=8YFLogxK
U2 - 10.1101/sqb.2019.84.039321
DO - 10.1101/sqb.2019.84.039321
M3 - Article
C2 - 32019864
AN - SCOPUS:85088608375
SN - 0091-7451
VL - 84
SP - 179
EP - 183
JO - Cold Spring Harbor Symposia on Quantitative Biology
JF - Cold Spring Harbor Symposia on Quantitative Biology
ER -