TY - JOUR
T1 - Host cell-dependent regulation of growth transformation-associated Epstein-Barr virus antigens in somatic cell hybrids
AU - Contreras-Salazar, B.
AU - Klein, G.
AU - Masucci, M. G.
PY - 1989
Y1 - 1989
N2 - We have analyzed the expression of the three major known growth transformation-associated Epstein-Barr virus (EBV) proteins, EBNA-1, EBNA-2, and latent membrane protein (LMP), in a series of somatic cell hybrids derived from the fusion of EBV-carrying Burkitt lymphoma (BL) lines with EBV-positive or EBV-negative B-cell lines. Independently of the cell phenotype, EBNA-1 was invariably coexpressed in all EBV-carrying hybrids. In hybrids between EBV-carrying, LMP-positive and LMP-negative Burkitt lymphoma lines, LMP was expressed, indicating positive control. Two EBV-negative lymphoma lines, Ramos and BJAB, differed in their ability to express LMP after B95-8 virus-induced conversion and after hybridization with Raji cells. BJAB was permissive while Ramos was nonpermissive for LMP, although both expressed EBNA-2. The EBNA-2-deleted P3HR-1 virus gave the same pattern of LMP expression in these two cells. Our findings indicate that the exression of EBNA-1, EBNA-2, and LMP is regulated by independent mechanisms.
AB - We have analyzed the expression of the three major known growth transformation-associated Epstein-Barr virus (EBV) proteins, EBNA-1, EBNA-2, and latent membrane protein (LMP), in a series of somatic cell hybrids derived from the fusion of EBV-carrying Burkitt lymphoma (BL) lines with EBV-positive or EBV-negative B-cell lines. Independently of the cell phenotype, EBNA-1 was invariably coexpressed in all EBV-carrying hybrids. In hybrids between EBV-carrying, LMP-positive and LMP-negative Burkitt lymphoma lines, LMP was expressed, indicating positive control. Two EBV-negative lymphoma lines, Ramos and BJAB, differed in their ability to express LMP after B95-8 virus-induced conversion and after hybridization with Raji cells. BJAB was permissive while Ramos was nonpermissive for LMP, although both expressed EBNA-2. The EBNA-2-deleted P3HR-1 virus gave the same pattern of LMP expression in these two cells. Our findings indicate that the exression of EBNA-1, EBNA-2, and LMP is regulated by independent mechanisms.
UR - http://www.scopus.com/inward/record.url?scp=0024369747&partnerID=8YFLogxK
U2 - 10.1128/jvi.63.6.2768-2772.1989
DO - 10.1128/jvi.63.6.2768-2772.1989
M3 - Article
C2 - 2542588
AN - SCOPUS:0024369747
SN - 0022-538X
VL - 63
SP - 2768
EP - 2772
JO - Journal of Virology
JF - Journal of Virology
IS - 6
ER -