Homer1 alternative splicing is regulated by gonadotropin-releasing hormone and modulates gonadotropin gene expression

Hypothalamic gonadotropin-releasing hormone (GnRH) plays a critical role in reproductive physiology by regulating folliclestimulating hormone (FSH) and luteinizing hormone (LH) gene expression in the pituitary. Analysis of gonadotrope deep-sequencing data identified a global regulation of pre-mRNA splicing by GnRH. Homer1, a gene encoding a postsynaptic density scaffolding protein, was selected for further study. Homer1 expresses a short splice form, Homer1a, and more-abundant long transcripts Homer1b/c. GnRH induced a modest increase in Homer1b/c expression and a dramatic increase in the Homer1a splice form. G protein knockdown studies suggested that the Homer1 induction, but not the regulated splicing, was Gαq/11 dependent. Phosphorylation of the splicing regulator SRp20 was found to be induced by GnRH. SRp20 depletion attenuated the GnRH-induced increase in the Homer1a-to-Homer1b/c ratio and modulated the effects of GnRH on FSHß and LHß expression. Homer1 gene knockdown resulted in increased GnRH-induced FSHß and LHß transcript levels. Furthermore, splice-form-specific reduction of Homer1b/c increased both FSHß and LHß mRNA induction, whereas reduction of Homer1a had the opposite effect on FSHß induction. These results indicate that the regulation of Homer1 splicing by GnRH contributes to gonadotropin gene control.