Histochemical assay of estrogen and progesterone receptors in breast cancer: Correlation with biochemical assays and patients' response to endocrine therapies

Louis P. Pertschuk; Ellis H. Tobin; Eric Gaetjens; Anne C. Carter; George A. Degenshein; Norman D. Bloom; David J. Brigati

doi:10.1002/1097-0142(19801215)46:12+<2896::AID-CNCR2820461431>3.0.CO;2-9

Histochemical assay of estrogen and progesterone receptors in breast cancer: Correlation with biochemical assays and patients' response to endocrine therapies

Louis P. Pertschuk, Ellis H. Tobin, Eric Gaetjens, Anne C. Carter, George A. Degenshein, Norman D. Bloom, David J. Brigati

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Abstract

Estrogen (ER) and progesterone (PgR) receptors were assayed by histochemistry in primary, recurrent, and metastatic breast cancer. Ligand‐conjugates composed of 17β‐estradiol and 11α‐hydroxyprogesterone covalently linked to bovine serum albumin and labelled with fluorescin isothiocyanate were employed. Results were compared with those of conventional biochemical receptor assays and correlated for ER in 92% of 314 tumors and for PgR in 86% of 86 specimens. ER and PgR determinations by both assay systems were correlated with clinical response to various endocrine therapies in 40 women with Stage IV disease. The histochemical assay enabled successful prediction of response in 80% of cases including eight which could not be fully analyzed biochemically.

Original language	English
Pages (from-to)	2896-2901
Number of pages	6
Journal	Cancer
Volume	46
Issue number	12 S
DOIs	https://doi.org/10.1002/1097-0142(19801215)46:12+<2896::AID-CNCR2820461431>3.0.CO;2-9
State	Published - 15 Dec 1980
Externally published	Yes

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10.1002/1097-0142(19801215)46:12+<2896::AID-CNCR2820461431>3.0.CO;2-9

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Pertschuk, L. P., Tobin, E. H., Gaetjens, E., Carter, A. C., Degenshein, G. A., Bloom, N. D., & Brigati, D. J. (1980). Histochemical assay of estrogen and progesterone receptors in breast cancer: Correlation with biochemical assays and patients' response to endocrine therapies. Cancer, 46(12 S), 2896-2901. https://doi.org/10.1002/1097-0142(19801215)46:12+<2896::AID-CNCR2820461431>3.0.CO;2-9

@article{146b99d9c95f42b892f8416af4ead1c1,

title = "Histochemical assay of estrogen and progesterone receptors in breast cancer: Correlation with biochemical assays and patients' response to endocrine therapies",

abstract = "Estrogen (ER) and progesterone (PgR) receptors were assayed by histochemistry in primary, recurrent, and metastatic breast cancer. Ligand‐conjugates composed of 17β‐estradiol and 11α‐hydroxyprogesterone covalently linked to bovine serum albumin and labelled with fluorescin isothiocyanate were employed. Results were compared with those of conventional biochemical receptor assays and correlated for ER in 92\% of 314 tumors and for PgR in 86\% of 86 specimens. ER and PgR determinations by both assay systems were correlated with clinical response to various endocrine therapies in 40 women with Stage IV disease. The histochemical assay enabled successful prediction of response in 80\% of cases including eight which could not be fully analyzed biochemically.",

author = "Pertschuk, \{Louis P.\} and Tobin, \{Ellis H.\} and Eric Gaetjens and Carter, \{Anne C.\} and Degenshein, \{George A.\} and Bloom, \{Norman D.\} and Brigati, \{David J.\}",

year = "1980",

month = dec,

day = "15",

doi = "10.1002/1097-0142(19801215)46:12+<2896::AID-CNCR2820461431>3.0.CO;2-9",

language = "English",

volume = "46",

pages = "2896--2901",

journal = "Cancer",

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Pertschuk, LP, Tobin, EH, Gaetjens, E, Carter, AC, Degenshein, GA, Bloom, ND & Brigati, DJ 1980, 'Histochemical assay of estrogen and progesterone receptors in breast cancer: Correlation with biochemical assays and patients' response to endocrine therapies', Cancer, vol. 46, no. 12 S, pp. 2896-2901. https://doi.org/10.1002/1097-0142(19801215)46:12+<2896::AID-CNCR2820461431>3.0.CO;2-9

TY - JOUR

T1 - Histochemical assay of estrogen and progesterone receptors in breast cancer

T2 - Correlation with biochemical assays and patients' response to endocrine therapies

AU - Pertschuk, Louis P.

AU - Tobin, Ellis H.

AU - Gaetjens, Eric

AU - Carter, Anne C.

AU - Degenshein, George A.

AU - Bloom, Norman D.

AU - Brigati, David J.

PY - 1980/12/15

Y1 - 1980/12/15

N2 - Estrogen (ER) and progesterone (PgR) receptors were assayed by histochemistry in primary, recurrent, and metastatic breast cancer. Ligand‐conjugates composed of 17β‐estradiol and 11α‐hydroxyprogesterone covalently linked to bovine serum albumin and labelled with fluorescin isothiocyanate were employed. Results were compared with those of conventional biochemical receptor assays and correlated for ER in 92% of 314 tumors and for PgR in 86% of 86 specimens. ER and PgR determinations by both assay systems were correlated with clinical response to various endocrine therapies in 40 women with Stage IV disease. The histochemical assay enabled successful prediction of response in 80% of cases including eight which could not be fully analyzed biochemically.

AB - Estrogen (ER) and progesterone (PgR) receptors were assayed by histochemistry in primary, recurrent, and metastatic breast cancer. Ligand‐conjugates composed of 17β‐estradiol and 11α‐hydroxyprogesterone covalently linked to bovine serum albumin and labelled with fluorescin isothiocyanate were employed. Results were compared with those of conventional biochemical receptor assays and correlated for ER in 92% of 314 tumors and for PgR in 86% of 86 specimens. ER and PgR determinations by both assay systems were correlated with clinical response to various endocrine therapies in 40 women with Stage IV disease. The histochemical assay enabled successful prediction of response in 80% of cases including eight which could not be fully analyzed biochemically.

UR - https://www.scopus.com/pages/publications/0019203690

U2 - 10.1002/1097-0142(19801215)46:12+<2896::AID-CNCR2820461431>3.0.CO;2-9

DO - 10.1002/1097-0142(19801215)46:12+<2896::AID-CNCR2820461431>3.0.CO;2-9

M3 - Article

C2 - 6256056

AN - SCOPUS:0019203690

SN - 0008-543X

VL - 46

SP - 2896

EP - 2901

JO - Cancer

JF - Cancer

IS - 12 S

ER -

Histochemical assay of estrogen and progesterone receptors in breast cancer: Correlation with biochemical assays and patients' response to endocrine therapies

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