Highly sensitive and simple method for determination of free 3-methoxy-4-hydroxyphenylglycol in plasma by high-performance liquid chromatography using a sep-pak alumina b cartridge

Ren Kui Yang; Gordon Campbell; Kenneth L. Davis

doi:10.1080/01483918808076791

Highly sensitive and simple method for determination of free 3-methoxy-4-hydroxyphenylglycol in plasma by high-performance liquid chromatography using a sep-pak alumina b cartridge

Ren Kui Yang, Gordon Campbell, Kenneth L. Davis

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

A novel sample clean-up procedure for the determination of free 3-methoxy-4-hydroxyphenylglycol (MHPG) in plasma is described. MHPG was purified with Sep-Pak alumina B cartridge followed by ethyl acetate extraction from the Cartridge. High-performance liquid chromatography with amperometric detection was used for separation and detection of MHPG and the internal standard 3-ethoxy-4-hydroxyphenylglycol (EHPG). This method provided good, clean chromatograms with base-line separation of the appropriate peaks. This technique is sensitive, reliable and less time-consuming than other methods. Thus, only 0.5 ml of plasma is needed and.

Original language	English
Pages (from-to)	3223-3231
Number of pages	9
Journal	Journal of Liquid Chromatography
Volume	11
Issue number	15
DOIs	https://doi.org/10.1080/01483918808076791
State	Published - 1 Nov 1988
Externally published	Yes

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10.1080/01483918808076791

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@article{63b7c71dad4a43a1bf13a990b3ca252c,

title = "Highly sensitive and simple method for determination of free 3-methoxy-4-hydroxyphenylglycol in plasma by high-performance liquid chromatography using a sep-pak alumina b cartridge",

abstract = "A novel sample clean-up procedure for the determination of free 3-methoxy-4-hydroxyphenylglycol (MHPG) in plasma is described. MHPG was purified with Sep-Pak alumina B cartridge followed by ethyl acetate extraction from the Cartridge. High-performance liquid chromatography with amperometric detection was used for separation and detection of MHPG and the internal standard 3-ethoxy-4-hydroxyphenylglycol (EHPG). This method provided good, clean chromatograms with base-line separation of the appropriate peaks. This technique is sensitive, reliable and less time-consuming than other methods. Thus, only 0.5 ml of plasma is needed and.",

author = "Yang, \{Ren Kui\} and Gordon Campbell and Davis, \{Kenneth L.\}",

note = "Funding Information: The authors wish to thank Dr. Kenneth L. Kirk of National Institutes of Health for the kind donation of the internal standard EHPG, and to thank Dr. Peter Knott for review of the text. We are grateful to Vivian L Brown for typing the manuscript. This work was supported by a grant from the NIH, \#AG05138-03.",

year = "1988",

month = nov,

day = "1",

doi = "10.1080/01483918808076791",

language = "English",

volume = "11",

pages = "3223--3231",

journal = "Journal of Liquid Chromatography",

issn = "0148-3919",

publisher = "Taylor and Francis Ltd.",

number = "15",

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Highly sensitive and simple method for determination of free 3-methoxy-4-hydroxyphenylglycol in plasma by high-performance liquid chromatography using a sep-pak alumina b cartridge. / Yang, Ren Kui; Campbell, Gordon; Davis, Kenneth L.
In: Journal of Liquid Chromatography, Vol. 11, No. 15, 01.11.1988, p. 3223-3231.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Highly sensitive and simple method for determination of free 3-methoxy-4-hydroxyphenylglycol in plasma by high-performance liquid chromatography using a sep-pak alumina b cartridge

AU - Yang, Ren Kui

AU - Campbell, Gordon

AU - Davis, Kenneth L.

N1 - Funding Information: The authors wish to thank Dr. Kenneth L. Kirk of National Institutes of Health for the kind donation of the internal standard EHPG, and to thank Dr. Peter Knott for review of the text. We are grateful to Vivian L Brown for typing the manuscript. This work was supported by a grant from the NIH, #AG05138-03.

PY - 1988/11/1

Y1 - 1988/11/1

N2 - A novel sample clean-up procedure for the determination of free 3-methoxy-4-hydroxyphenylglycol (MHPG) in plasma is described. MHPG was purified with Sep-Pak alumina B cartridge followed by ethyl acetate extraction from the Cartridge. High-performance liquid chromatography with amperometric detection was used for separation and detection of MHPG and the internal standard 3-ethoxy-4-hydroxyphenylglycol (EHPG). This method provided good, clean chromatograms with base-line separation of the appropriate peaks. This technique is sensitive, reliable and less time-consuming than other methods. Thus, only 0.5 ml of plasma is needed and.

AB - A novel sample clean-up procedure for the determination of free 3-methoxy-4-hydroxyphenylglycol (MHPG) in plasma is described. MHPG was purified with Sep-Pak alumina B cartridge followed by ethyl acetate extraction from the Cartridge. High-performance liquid chromatography with amperometric detection was used for separation and detection of MHPG and the internal standard 3-ethoxy-4-hydroxyphenylglycol (EHPG). This method provided good, clean chromatograms with base-line separation of the appropriate peaks. This technique is sensitive, reliable and less time-consuming than other methods. Thus, only 0.5 ml of plasma is needed and.

UR - https://www.scopus.com/pages/publications/0024232073

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DO - 10.1080/01483918808076791

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VL - 11

SP - 3223

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JO - Journal of Liquid Chromatography

JF - Journal of Liquid Chromatography

IS - 15

ER -

Highly sensitive and simple method for determination of free 3-methoxy-4-hydroxyphenylglycol in plasma by high-performance liquid chromatography using a sep-pak alumina b cartridge

Abstract

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