High-throughput transfection of interfering RNA into a 3D cell-culture chip

Haiyuan Zhang; Moo Yeal Lee; Michael G. Hogg; Jonathan S. Dordick; Susan T. Sharfstein

doi:10.1002/smll.201102205

High-throughput transfection of interfering RNA into a 3D cell-culture chip

Haiyuan Zhang, Moo Yeal Lee, Michael G. Hogg, Jonathan S. Dordick, Susan T. Sharfstein

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A method for high-throughput retroviral transfection of genes and interfering RNA into 3D cell-culture microarrays is described. 3D cultures more closely mimic the in vivo cellular milieu, thus providing cellular responses to genetic manipulation more similar to the in vivo situation than 2D cultures. This technique is applied to transfect several "toxic" short-hairpin RNAs (shRNAs) into 3D cell cultures. It is demonstrated that the toxicity is similar to that obtained by conventional (non-high-throughput) retroviral transfection of cells grown in similar 3D culture microarrays.

Original language	English
Pages (from-to)	2091-2098
Number of pages	8
Journal	Small
Volume	8
Issue number	13
DOIs	https://doi.org/10.1002/smll.201102205
State	Published - 9 Jul 2012
Externally published	Yes

Keywords

RNA
cell-culture chips
gene technology
high-throughput transfection
three-dimensional cell culture

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10.1002/smll.201102205

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abstract = "A method for high-throughput retroviral transfection of genes and interfering RNA into 3D cell-culture microarrays is described. 3D cultures more closely mimic the in vivo cellular milieu, thus providing cellular responses to genetic manipulation more similar to the in vivo situation than 2D cultures. This technique is applied to transfect several {"}toxic{"} short-hairpin RNAs (shRNAs) into 3D cell cultures. It is demonstrated that the toxicity is similar to that obtained by conventional (non-high-throughput) retroviral transfection of cells grown in similar 3D culture microarrays.",

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T1 - High-throughput transfection of interfering RNA into a 3D cell-culture chip

AU - Zhang, Haiyuan

AU - Lee, Moo Yeal

AU - Hogg, Michael G.

AU - Dordick, Jonathan S.

AU - Sharfstein, Susan T.

PY - 2012/7/9

Y1 - 2012/7/9

N2 - A method for high-throughput retroviral transfection of genes and interfering RNA into 3D cell-culture microarrays is described. 3D cultures more closely mimic the in vivo cellular milieu, thus providing cellular responses to genetic manipulation more similar to the in vivo situation than 2D cultures. This technique is applied to transfect several "toxic" short-hairpin RNAs (shRNAs) into 3D cell cultures. It is demonstrated that the toxicity is similar to that obtained by conventional (non-high-throughput) retroviral transfection of cells grown in similar 3D culture microarrays.

AB - A method for high-throughput retroviral transfection of genes and interfering RNA into 3D cell-culture microarrays is described. 3D cultures more closely mimic the in vivo cellular milieu, thus providing cellular responses to genetic manipulation more similar to the in vivo situation than 2D cultures. This technique is applied to transfect several "toxic" short-hairpin RNAs (shRNAs) into 3D cell cultures. It is demonstrated that the toxicity is similar to that obtained by conventional (non-high-throughput) retroviral transfection of cells grown in similar 3D culture microarrays.

KW - RNA

KW - cell-culture chips

KW - gene technology

KW - high-throughput transfection

KW - three-dimensional cell culture

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SN - 1613-6810

VL - 8

SP - 2091

EP - 2098

JO - Small

JF - Small

IS - 13

ER -

High-throughput transfection of interfering RNA into a 3D cell-culture chip

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Keywords

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