High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform

Gregory J. Nierode; Brian C. Perea; Sean K. McFarland; Jorge F. Pascoal; Douglas S. Clark; David V. Schaffer; Jonathan S. Dordick

doi:10.1016/j.stemcr.2016.10.001

High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform

Gregory J. Nierode
, Brian C. Perea
, Sean K. McFarland
, Jorge F. Pascoal
, Douglas S. Clark
, David V. Schaffer
, Jonathan S. Dordick

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

A 3D cell culture chip was used for high-throughput screening of a human neural progenitor cell line. The differential toxicity of 24 compounds was determined on undifferentiated and differentiating NPCs. Five compounds led to significant differences in IC₅₀ values between undifferentiated and differentiating cultures. This platform has potential use in phenotypic screening to elucidate molecular toxicology on human stem cells.

Original language	English
Pages (from-to)	970-982
Number of pages	13
Journal	Stem Cell Reports
Volume	7
Issue number	5
DOIs	https://doi.org/10.1016/j.stemcr.2016.10.001
State	Published - 8 Nov 2016
Externally published	Yes

Keywords

high-content screening
high-throughput microarray
human neural stem cells
neurotoxicity
screening
three-dimensional cell culture

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10.1016/j.stemcr.2016.10.001

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title = "High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform",

abstract = "A 3D cell culture chip was used for high-throughput screening of a human neural progenitor cell line. The differential toxicity of 24 compounds was determined on undifferentiated and differentiating NPCs. Five compounds led to significant differences in IC50 values between undifferentiated and differentiating cultures. This platform has potential use in phenotypic screening to elucidate molecular toxicology on human stem cells.",

keywords = "high-content screening, high-throughput microarray, human neural stem cells, neurotoxicity, screening, three-dimensional cell culture",

author = "Nierode, \{Gregory J.\} and Perea, \{Brian C.\} and McFarland, \{Sean K.\} and Pascoal, \{Jorge F.\} and Clark, \{Douglas S.\} and Schaffer, \{David V.\} and Dordick, \{Jonathan S.\}",

note = "Publisher Copyright: {\textcopyright} 2016 The Author(s)",

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T1 - High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform

AU - Nierode, Gregory J.

AU - Perea, Brian C.

AU - McFarland, Sean K.

AU - Pascoal, Jorge F.

AU - Clark, Douglas S.

AU - Schaffer, David V.

AU - Dordick, Jonathan S.

PY - 2016/11/8

Y1 - 2016/11/8

N2 - A 3D cell culture chip was used for high-throughput screening of a human neural progenitor cell line. The differential toxicity of 24 compounds was determined on undifferentiated and differentiating NPCs. Five compounds led to significant differences in IC50 values between undifferentiated and differentiating cultures. This platform has potential use in phenotypic screening to elucidate molecular toxicology on human stem cells.

AB - A 3D cell culture chip was used for high-throughput screening of a human neural progenitor cell line. The differential toxicity of 24 compounds was determined on undifferentiated and differentiating NPCs. Five compounds led to significant differences in IC50 values between undifferentiated and differentiating cultures. This platform has potential use in phenotypic screening to elucidate molecular toxicology on human stem cells.

KW - high-content screening

KW - high-throughput microarray

KW - human neural stem cells

KW - neurotoxicity

KW - screening

KW - three-dimensional cell culture

UR - https://www.scopus.com/pages/publications/84994552896

U2 - 10.1016/j.stemcr.2016.10.001

DO - 10.1016/j.stemcr.2016.10.001

M3 - Article

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SN - 2213-6711

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EP - 982

JO - Stem Cell Reports

JF - Stem Cell Reports

IS - 5

ER -

High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform

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Keywords

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