High-Throughput Cell Death Assays with Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling (SPARKL)

Jesse D. Gelles, Jerry E. Chipuk

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

High-throughput cytostatic and cell death assays are a critical component of pharmacological screens and mechanism-based interrogations into cellular biology. We developed a method for single-cell and population-level analyses using real-time kinetic labeling (abbreviated “SPARKL”) with non-toxic fluorescent probes and high-content live-cell imagers. The protocols herein detail the steps, specifics, and suggested utilization of the SPARKL method within several “label-and-go” zero-handling workflows. For complete details on the use and execution of this protocol, please refer to Gelles et al. (2019).

Original languageEnglish
Article number100034
JournalSTAR Protocols
Volume1
Issue number1
DOIs
StatePublished - 19 Jun 2020

Fingerprint

Dive into the research topics of 'High-Throughput Cell Death Assays with Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling (SPARKL)'. Together they form a unique fingerprint.

Cite this