High-performance liquid chromatographic determination of the calcium channel blocker nimodipine in monkey plasma

Mingxin Qian; James M. Gallo

doi:10.1016/0378-4347(92)80432-P

High-performance liquid chromatographic determination of the calcium channel blocker nimodipine in monkey plasma

Mingxin Qian, James M. Gallo

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29 Scopus citations

Abstract

A new high-performance liquid chromatographic (HPLC) assay was developed for the determination of nimodipine in monkey plasma. An ethyl acetate extraction procedure was employed with a reversed-phase HPLC separation for the analysis. Absolute recovery of nimodipine from plasma was over 95% with a lower limit of quantitation of 10 ng/ml. This method was applied to a preliminary pharmacokinetic study in which 0.25 mg/kg nimodipine was administered intravenously to three monkeys. Protein binding and stability of nimodipine in monkey plasma were also examined. The pharmacokinetic parameters of nimodipine in monkeys were similar to those obtained in humans and indicate that monkeys are an appropriate animal model for further pharmacokinetic investigations.

Original language	English
Pages (from-to)	316-320
Number of pages	5
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	578
Issue number	2
DOIs	https://doi.org/10.1016/0378-4347(92)80432-P
State	Published - 24 Jul 1992
Externally published	Yes

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title = "High-performance liquid chromatographic determination of the calcium channel blocker nimodipine in monkey plasma",

abstract = "A new high-performance liquid chromatographic (HPLC) assay was developed for the determination of nimodipine in monkey plasma. An ethyl acetate extraction procedure was employed with a reversed-phase HPLC separation for the analysis. Absolute recovery of nimodipine from plasma was over 95% with a lower limit of quantitation of 10 ng/ml. This method was applied to a preliminary pharmacokinetic study in which 0.25 mg/kg nimodipine was administered intravenously to three monkeys. Protein binding and stability of nimodipine in monkey plasma were also examined. The pharmacokinetic parameters of nimodipine in monkeys were similar to those obtained in humans and indicate that monkeys are an appropriate animal model for further pharmacokinetic investigations.",

author = "Mingxin Qian and Gallo, {James M.}",

note = "Funding Information: This project was supportedb y Food and Drug Administration Contract 223-89-3806.",

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AU - Gallo, James M.

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PY - 1992/7/24

Y1 - 1992/7/24

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AB - A new high-performance liquid chromatographic (HPLC) assay was developed for the determination of nimodipine in monkey plasma. An ethyl acetate extraction procedure was employed with a reversed-phase HPLC separation for the analysis. Absolute recovery of nimodipine from plasma was over 95% with a lower limit of quantitation of 10 ng/ml. This method was applied to a preliminary pharmacokinetic study in which 0.25 mg/kg nimodipine was administered intravenously to three monkeys. Protein binding and stability of nimodipine in monkey plasma were also examined. The pharmacokinetic parameters of nimodipine in monkeys were similar to those obtained in humans and indicate that monkeys are an appropriate animal model for further pharmacokinetic investigations.

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High-performance liquid chromatographic determination of the calcium channel blocker nimodipine in monkey plasma

Abstract

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