Abstract
OBJECTIVE: To develop an HPLC method for the determination of floxuridine in human serum. METHODS: With metronidazole as the internal standard, 200 μL of serum was extracted by n-propyl alcohol/methyl t- butyl ether in a two-step extraction. The organic layer was evaporated under nitrogen stream and the residual was reconstituted with the mobile plase. A Shim-Pack CLC-ODS column was selected and the mobile phase was consisted of acetonitrile-phosphate buffer-water (75 : 100 : 900) at a flow rate of 0.6 mL·min-1. The detection wavelength was 268 nm. RESULTS: A linearity was obtained from 0.005 to 0.5 mg·L-1 of floxuridine in serum with a good correlation coefficient (r = 0.9999, n = 8). The intra-run and inter-run coefficients of variation were less than 4.09%. The mean recoveries were 103.00%, 107.00% and 100.88% for the low, middle and high concentrations of check samples, respectively. The limit of detection was 0.001 mg·L-1. CONCLUSION: The method was sensitive, specific and simple. It is suitable for clinical pharmacokinetic study.
Original language | English |
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Pages (from-to) | 118-120 |
Number of pages | 3 |
Journal | Chinese Pharmaceutical Journal |
Volume | 36 |
Issue number | 2 |
State | Published - 2001 |
Externally published | Yes |
Keywords
- Floxuridine
- HPLC
- Serum