High-frequency transfection of CHO cells using polybrene

William G. Chaney, Daniel R. Howard, Jeffrey W. Pollard, Sandra Sallustio, Pamela Stanley

Research output: Contribution to journalArticlepeer-review

126 Scopus citations


High-frequency transfection of CHO cells has been achieved for several plasmids, a cosmid library, and genomic DNA using Polybrene and dimethyl sulfoxide. All plasmid transfectants examined were stable and exhibited plasmid sequences in genomic DNA. The method is simple, reproducible, and succeeded with several independent CHO clones in the presence or the absence of carrier DNA, even at very low concentrations of plasmid DNA.

Original languageEnglish
Pages (from-to)237-244
Number of pages8
JournalSomatic Cell and Molecular Genetics
Issue number3
StatePublished - May 1986
Externally publishedYes


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