High frequency of autoantibodies bearing cross-reactive idiotopes among hybridomas using V(H)7183 genes prepared from normal and autoimmune murine strains

B. Bellon; A. Manheimer-Lory; M. Monestier; T. Moran; A. Dimitriu-Bona; F. Alt; C. Bona

doi:10.1172/JCI112917

High frequency of autoantibodies bearing cross-reactive idiotopes among hybridomas using V(H)7183 genes prepared from normal and autoimmune murine strains

B. Bellon, A. Manheimer-Lory, M. Monestier, T. Moran, A. Dimitriu-Bona, F. Alt, C. Bona

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Abstract

Hybridomas obtained by in vitro stimulation with lipopolysaccharides (LPS) of BALB/c, MRL/lpr, and NZB splenocytes were selected for expression of V(H)7183 by hybridization using slot blotting. Northern blot analysis showed that the majority of hybrids produce a full length message complementary to the V(H)7183 probe. The frequency of V(H)7183 hybridomas was significantly higher in NZB mice as compared with BALB/c mice. Using multiple binding assays, 60% of the total antibodies encoded by V(H)7183 were specific for self-epitopes. Finally, the vast majority express cross-reactive idiotypes borne by auto-antibodies of various specificities.

Original language	English
Pages (from-to)	1044-1053
Number of pages	10
Journal	Journal of Clinical Investigation
Volume	79
Issue number	4
DOIs	https://doi.org/10.1172/JCI112917
State	Published - 1987

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title = "High frequency of autoantibodies bearing cross-reactive idiotopes among hybridomas using V(H)7183 genes prepared from normal and autoimmune murine strains",

abstract = "Hybridomas obtained by in vitro stimulation with lipopolysaccharides (LPS) of BALB/c, MRL/lpr, and NZB splenocytes were selected for expression of V(H)7183 by hybridization using slot blotting. Northern blot analysis showed that the majority of hybrids produce a full length message complementary to the V(H)7183 probe. The frequency of V(H)7183 hybridomas was significantly higher in NZB mice as compared with BALB/c mice. Using multiple binding assays, 60% of the total antibodies encoded by V(H)7183 were specific for self-epitopes. Finally, the vast majority express cross-reactive idiotypes borne by auto-antibodies of various specificities.",

author = "B. Bellon and A. Manheimer-Lory and M. Monestier and T. Moran and A. Dimitriu-Bona and F. Alt and C. Bona",

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T1 - High frequency of autoantibodies bearing cross-reactive idiotopes among hybridomas using V(H)7183 genes prepared from normal and autoimmune murine strains

AU - Bellon, B.

AU - Manheimer-Lory, A.

AU - Monestier, M.

AU - Moran, T.

AU - Dimitriu-Bona, A.

AU - Alt, F.

AU - Bona, C.

PY - 1987

Y1 - 1987

N2 - Hybridomas obtained by in vitro stimulation with lipopolysaccharides (LPS) of BALB/c, MRL/lpr, and NZB splenocytes were selected for expression of V(H)7183 by hybridization using slot blotting. Northern blot analysis showed that the majority of hybrids produce a full length message complementary to the V(H)7183 probe. The frequency of V(H)7183 hybridomas was significantly higher in NZB mice as compared with BALB/c mice. Using multiple binding assays, 60% of the total antibodies encoded by V(H)7183 were specific for self-epitopes. Finally, the vast majority express cross-reactive idiotypes borne by auto-antibodies of various specificities.

AB - Hybridomas obtained by in vitro stimulation with lipopolysaccharides (LPS) of BALB/c, MRL/lpr, and NZB splenocytes were selected for expression of V(H)7183 by hybridization using slot blotting. Northern blot analysis showed that the majority of hybrids produce a full length message complementary to the V(H)7183 probe. The frequency of V(H)7183 hybridomas was significantly higher in NZB mice as compared with BALB/c mice. Using multiple binding assays, 60% of the total antibodies encoded by V(H)7183 were specific for self-epitopes. Finally, the vast majority express cross-reactive idiotypes borne by auto-antibodies of various specificities.

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High frequency of autoantibodies bearing cross-reactive idiotopes among hybridomas using V(H)7183 genes prepared from normal and autoimmune murine strains

Abstract

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