High-efficiency formation of influenza virus transfectants

M. Enami, P. Palese

Research output: Contribution to journalComment/debate

1 Scopus citations


cDNA-derived RNAs were introduced into the genomes of influenza viruses by using an improved ribonucleoprotein (RNP) transfection protocol. Up to 105 viral transfectants with a novel neuraminidase gene could be obtained by using a 35-mm dish (106 cells) for RNP transfection. In addition to genes coding for surface proteins (hemagglutinin and neuraminidase), we also exchanged a gene coding for nonsurface proteins. The cDNA-derived influenza A/PR/8/34 virus NS gene was introduced into a temperature-sensitive mutant with a defect in this gene. We suggest that the term influenza virus transfectant be used for those viruses which are made by RNP transfection with cDNA-derived RNA.

Original languageEnglish
Pages (from-to)2711-2713
Number of pages3
JournalJournal of Virology
Issue number5
StatePublished - 1991


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