Abstract
cDNA-derived RNAs were introduced into the genomes of influenza viruses by using an improved ribonucleoprotein (RNP) transfection protocol. Up to 105 viral transfectants with a novel neuraminidase gene could be obtained by using a 35-mm dish (106 cells) for RNP transfection. In addition to genes coding for surface proteins (hemagglutinin and neuraminidase), we also exchanged a gene coding for nonsurface proteins. The cDNA-derived influenza A/PR/8/34 virus NS gene was introduced into a temperature-sensitive mutant with a defect in this gene. We suggest that the term influenza virus transfectant be used for those viruses which are made by RNP transfection with cDNA-derived RNA.
| Original language | English |
|---|---|
| Pages (from-to) | 2711-2713 |
| Number of pages | 3 |
| Journal | Journal of Virology |
| Volume | 65 |
| Issue number | 5 |
| State | Published - May 1991 |