High-efficiency FLP and ΦC31 site-specific recombination in mammalian cells

Christopher S. Raymond; Philippe Soriano

doi:10.1371/journal.pone.0000162

High-efficiency FLP and ΦC31 site-specific recombination in mammalian cells

Christopher S. Raymond, Philippe Soriano

Research output: Contribution to journal › Article › peer-review

306 Scopus citations

Abstract

DNA site-specific recombinases (SSRs) such as Cre, FLPe, and φC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities, efficient recombination in mammalian cells has only been observed with Cre recombinase. Here we report the de novo synthesis of mouse codon-optimized FLP (FLPo) and ΦC31 (ΦC31o) SSRs, which result in recombination efficiencies similar to Cre.

Original language	English
Article number	e162
Journal	PLoS ONE
Volume	2
Issue number	1
DOIs	https://doi.org/10.1371/journal.pone.0000162
State	Published - 17 Jan 2007
Externally published	Yes

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abstract = "DNA site-specific recombinases (SSRs) such as Cre, FLPe, and φC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities, efficient recombination in mammalian cells has only been observed with Cre recombinase. Here we report the de novo synthesis of mouse codon-optimized FLP (FLPo) and ΦC31 (ΦC31o) SSRs, which result in recombination efficiencies similar to Cre.",

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High-efficiency FLP and ΦC31 site-specific recombination in mammalian cells

Abstract

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