High-coverage, long-read sequencing of Han Chinese trio reference samples

Ying Chih Wang, Nathan D. Olson, Gintaras Deikus, Hardik Shah, Aaron M. Wenger, Jonathan Trow, Chunlin Xiao, Stephen Sherry, Marc L. Salit, Justin M. Zook, Melissa Smith, Robert Sebra

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9 Scopus citations

Abstract

Single-molecule long-read sequencing datasets were generated for a son-father-mother trio of Han Chinese descent that is part of the Genome in a Bottle (GIAB) consortium portfolio. The dataset was generated using the Pacific Biosciences Sequel System. The son and each parent were sequenced to an average coverage of 60 and 30, respectively, with N50 subread lengths between 16 and 18 kb. Raw reads and reads aligned to both the GRCh37 and GRCh38 are available at the NCBI GIAB ftp site (ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/data/ChineseTrio/). The GRCh38 aligned read data are archived in NCBI SRA (SRX4739017, SRX4739121, and SRX4739122). This dataset is available for anyone to develop and evaluate long-read bioinformatics methods.

Original languageEnglish
Article number91
JournalScientific data
Volume6
Issue number1
DOIs
StatePublished - 1 Dec 2019

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