TY - JOUR
T1 - High content image analysis reveals function of miR-124 upstream of Vimentin in regulating motor neuron mitochondria
AU - Yardeni, Tal
AU - Fine, Raquel
AU - Joshi, Yuvraj
AU - Gradus-Pery, Tal
AU - Kozer, Noga
AU - Reichenstein, Irit
AU - Yanowski, Eran
AU - Nevo, Shir
AU - Weiss-Tishler, Hila
AU - Eisenberg-Bord, Michal
AU - Shalit, Tal
AU - Plotnikov, Alexander
AU - Barr, Haim M.
AU - Perlson, Eran
AU - Hornstein, Eran
N1 - Publisher Copyright:
© 2017 The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - microRNAs (miRNAs) are critical for neuronal function and their dysregulation is repeatedly observed in neurodegenerative diseases. Here, we implemented high content image analysis for investigating the impact of several miRNAs in mouse primary motor neurons. This survey directed our attention to the neuron-specific miR-124, which controls axonal morphology. By performing next generation sequencing analysis and molecular studies, we characterized novel roles for miR-124 in control of mitochondria localization and function. We further demonstrated that the intermediate filament Vimentin is a key target of miR-124 in this system. Our data establishes a new pathway for control of mitochondria function in motor neurons, revealing the value of a neuron-specific miRNA gene as a mechanism for the re-shaping of otherwise ubiquitously-expressed intermediate filament network, upstream of mitochondria activity and cellular metabolism.
AB - microRNAs (miRNAs) are critical for neuronal function and their dysregulation is repeatedly observed in neurodegenerative diseases. Here, we implemented high content image analysis for investigating the impact of several miRNAs in mouse primary motor neurons. This survey directed our attention to the neuron-specific miR-124, which controls axonal morphology. By performing next generation sequencing analysis and molecular studies, we characterized novel roles for miR-124 in control of mitochondria localization and function. We further demonstrated that the intermediate filament Vimentin is a key target of miR-124 in this system. Our data establishes a new pathway for control of mitochondria function in motor neurons, revealing the value of a neuron-specific miRNA gene as a mechanism for the re-shaping of otherwise ubiquitously-expressed intermediate filament network, upstream of mitochondria activity and cellular metabolism.
UR - http://www.scopus.com/inward/record.url?scp=85042783147&partnerID=8YFLogxK
U2 - 10.1038/s41598-017-17878-x
DO - 10.1038/s41598-017-17878-x
M3 - Article
C2 - 29311649
AN - SCOPUS:85042783147
SN - 2045-2322
VL - 8
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 59
ER -