Heterotransplantability of human cell lines derived from leukemia and lymphomas into immunologically tolerant rats

Rats were inoculated intravenously with living J‐111 cells on the day of birth to make them immunologically tolerant to human cell antigens. The subcutaneous transplantability of 17 human cell lines was tested in these rats at age 1‐3 weeks. Eight of the cell lines were derived from Burkitt's lymphomas (EB 1, EB 2, EB 3, Kudi, Jijoye, Ogun, Raji, SL‐1) one from reticulum cell sarcoma (SK‐RCS1), 6 from leukemia (SK‐L2 SK‐L4, SK‐L6, SK‐L7, SK‐L9, SK‐L10), one from a normal lymph node (SK‐LN1), and one from infectious mononucleosis (C566). Fourteen of these cell lines, including the 2 lines derived from nonmalignant sources, grew as subcutaneous nodules in some of the recipients. The most frequent and largest nodules were produced by lines EB 2 and EB 3 from Burkitt tumors, lines SK‐L2 and SK‐L4 from acute leukemia, and the 2 lines of nonmalignant origin (SKLN1 and C566). More of the cell lines grew subcutaneously in these immunologically tolerant rats than grew on intravenous injection into newborn rats in a previously reported study.