Heterogeneity of Human Peripheral Blood Mononuclear Cells Detected by Monoclonal Antibodies to Monomorphic Determinants of Human Ia Antigens

P. Marder; Ann Hinson; C. Russo; S. Ferrone; E. Ades

doi:10.1016/S0171-2985(84)80079-0

Heterogeneity of Human Peripheral Blood Mononuclear Cells Detected by Monoclonal Antibodies to Monomorphic Determinants of Human Ia Antigens

P. Marder, Ann Hinson, C. Russo, S. Ferrone, E. Ades

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The reactivity patterns of several monoclonal antibodies specific for monomorphic determinants of human Ia antigens were studied using flow cytometric techniques. We observed differential reactivity of these antibodies with human lymphoid cell lines, normal fresh human mononuclear cells, and lymphoblasts from PHA-activated cultures. The molecular heterogeneity of Ia antigens previously identified with immunochemical techniques was accompanied by heterogeneity of cell surface expression as identified by an immunofluorescent probe. The determinants identified by these anti-Ia monoclonal anti bodies may provide useful markers in the isolation of cellular subpopulations responsive in the immune system.

Original language	English
Pages (from-to)	483-494
Number of pages	12
Journal	Immunobiology
Volume	167
Issue number	5
DOIs	https://doi.org/10.1016/S0171-2985(84)80079-0
State	Published - 1984
Externally published	Yes

Keywords

FBS
HBSAZ
Hanks' balanced salt solution with added serum and azide
MoAb
PBL
PHA
fetal bovine serum
human peripheral blood mononuclear leucocytes
monoclonal antibody
phytohemagglutinin

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title = "Heterogeneity of Human Peripheral Blood Mononuclear Cells Detected by Monoclonal Antibodies to Monomorphic Determinants of Human Ia Antigens",

abstract = "The reactivity patterns of several monoclonal antibodies specific for monomorphic determinants of human Ia antigens were studied using flow cytometric techniques. We observed differential reactivity of these antibodies with human lymphoid cell lines, normal fresh human mononuclear cells, and lymphoblasts from PHA-activated cultures. The molecular heterogeneity of Ia antigens previously identified with immunochemical techniques was accompanied by heterogeneity of cell surface expression as identified by an immunofluorescent probe. The determinants identified by these anti-Ia monoclonal anti bodies may provide useful markers in the isolation of cellular subpopulations responsive in the immune system.",

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year = "1984",

doi = "10.1016/S0171-2985(84)80079-0",

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volume = "167",

pages = "483--494",

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T1 - Heterogeneity of Human Peripheral Blood Mononuclear Cells Detected by Monoclonal Antibodies to Monomorphic Determinants of Human Ia Antigens

AU - Marder, P.

AU - Hinson, Ann

AU - Russo, C.

AU - Ferrone, S.

AU - Ades, E.

PY - 1984

Y1 - 1984

N2 - The reactivity patterns of several monoclonal antibodies specific for monomorphic determinants of human Ia antigens were studied using flow cytometric techniques. We observed differential reactivity of these antibodies with human lymphoid cell lines, normal fresh human mononuclear cells, and lymphoblasts from PHA-activated cultures. The molecular heterogeneity of Ia antigens previously identified with immunochemical techniques was accompanied by heterogeneity of cell surface expression as identified by an immunofluorescent probe. The determinants identified by these anti-Ia monoclonal anti bodies may provide useful markers in the isolation of cellular subpopulations responsive in the immune system.

AB - The reactivity patterns of several monoclonal antibodies specific for monomorphic determinants of human Ia antigens were studied using flow cytometric techniques. We observed differential reactivity of these antibodies with human lymphoid cell lines, normal fresh human mononuclear cells, and lymphoblasts from PHA-activated cultures. The molecular heterogeneity of Ia antigens previously identified with immunochemical techniques was accompanied by heterogeneity of cell surface expression as identified by an immunofluorescent probe. The determinants identified by these anti-Ia monoclonal anti bodies may provide useful markers in the isolation of cellular subpopulations responsive in the immune system.

KW - FBS

KW - HBSAZ

KW - Hanks' balanced salt solution with added serum and azide

KW - MoAb

KW - PBL

KW - PHA

KW - fetal bovine serum

KW - human peripheral blood mononuclear leucocytes

KW - monoclonal antibody

KW - phytohemagglutinin

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DO - 10.1016/S0171-2985(84)80079-0

M3 - Article

AN - SCOPUS:0021711032

SN - 0171-2985

VL - 167

SP - 483

EP - 494

JO - Immunobiology

JF - Immunobiology

IS - 5

ER -

Heterogeneity of Human Peripheral Blood Mononuclear Cells Detected by Monoclonal Antibodies to Monomorphic Determinants of Human Ia Antigens

Abstract

Keywords

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