Hepatic cells derived from induced pluripotent stem cells of pigtail macaques support hepatitis C virus infection

Marion Sourisseau, Orit Goldman, Wenqian He, Jennifer L. Gori, Hans Peter Kiem, Valerie Gouon-Evans, Matthew J. Evans

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

The narrow species tropism of hepatitis C virus (HCV) limits animal studies. We found that pigtail macaque (Macaca nemestrina) hepatic cells derived from induced pluripotent stem cells support the entire HCV life cycle, although infection efficiency was limited by defects in the HCV cell entry process. This block was overcome by either increasing occludin expression, complementing the cells with human CD81, or infecting them with a strain of HCV with less restricted requirements for CD81. Using this system, we can modify viral and host cell genetics to make pigtail macaques a suitable, clinically relevant model for the study of HCV infection.

Original languageEnglish
Pages (from-to)966-969.e7
JournalGastroenterology
Volume145
Issue number5
DOIs
StatePublished - Nov 2013

Keywords

  • Animal Model
  • Hepatitis C Virus
  • Monkey
  • Replication

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