Heparinase I acts on a synthetic heparin pentasaccharide corresponding to the antithrombin III binding site

Guangli Yu; Laurie LeBrun; Nur Sibel Gunay; Debra Hoppensteadt; Jeanine M. Walenga; Jawed Fareed; Robert J. Linhardt

doi:10.1016/S0049-3848(00)00368-6

Heparinase I acts on a synthetic heparin pentasaccharide corresponding to the antithrombin III binding site

Guangli Yu
, Laurie LeBrun
, Nur Sibel Gunay
, Debra Hoppensteadt
, Jeanine M. Walenga
, Jawed Fareed
, Robert J. Linhardt

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

A synthetic pentasaccharide, containing an intact antithrombin III (ATIII) binding site that is in clinical studies a specific antifactor Xa agent, serves as a substrate for a heparin lyase (heparinase I, EC 4.2.2.7) from Flavobacterium heparinum. Heparinase I, currently being assessed as a heparin reversal agent, also reverses the antifactor Xa activity of this synthetic pentasaccharide by breaking it down to inactive disaccharide and trisaccharide products.

Original language	English
Pages (from-to)	549-556
Number of pages	8
Journal	Thrombosis Research
Volume	100
Issue number	6
DOIs	https://doi.org/10.1016/S0049-3848(00)00368-6
State	Published - 15 Dec 2000
Externally published	Yes

Keywords

Heparin lyase
Heparinase
Neutralization
Pentasaccharide
Reversal

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10.1016/S0049-3848(00)00368-6

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@article{c6db366092c3450694db1e9f46cb505f,

title = "Heparinase I acts on a synthetic heparin pentasaccharide corresponding to the antithrombin III binding site",

abstract = "A synthetic pentasaccharide, containing an intact antithrombin III (ATIII) binding site that is in clinical studies a specific antifactor Xa agent, serves as a substrate for a heparin lyase (heparinase I, EC 4.2.2.7) from Flavobacterium heparinum. Heparinase I, currently being assessed as a heparin reversal agent, also reverses the antifactor Xa activity of this synthetic pentasaccharide by breaking it down to inactive disaccharide and trisaccharide products.",

keywords = "Heparin lyase, Heparinase, Neutralization, Pentasaccharide, Reversal",

author = "Guangli Yu and Laurie LeBrun and Gunay, \{Nur Sibel\} and Debra Hoppensteadt and Walenga, \{Jeanine M.\} and Jawed Fareed and Linhardt, \{Robert J.\}",

note = "Funding Information: This work was supported in part by National Institute of Health grants GM 38060 and HL52622 (RJL). ",

year = "2000",

month = dec,

day = "15",

doi = "10.1016/S0049-3848(00)00368-6",

language = "English",

volume = "100",

pages = "549--556",

journal = "Thrombosis Research",

issn = "0049-3848",

publisher = "Elsevier Ltd.",

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}

TY - JOUR

T1 - Heparinase I acts on a synthetic heparin pentasaccharide corresponding to the antithrombin III binding site

AU - Yu, Guangli

AU - LeBrun, Laurie

AU - Gunay, Nur Sibel

AU - Hoppensteadt, Debra

AU - Walenga, Jeanine M.

AU - Fareed, Jawed

AU - Linhardt, Robert J.

N1 - Funding Information: This work was supported in part by National Institute of Health grants GM 38060 and HL52622 (RJL).

PY - 2000/12/15

Y1 - 2000/12/15

N2 - A synthetic pentasaccharide, containing an intact antithrombin III (ATIII) binding site that is in clinical studies a specific antifactor Xa agent, serves as a substrate for a heparin lyase (heparinase I, EC 4.2.2.7) from Flavobacterium heparinum. Heparinase I, currently being assessed as a heparin reversal agent, also reverses the antifactor Xa activity of this synthetic pentasaccharide by breaking it down to inactive disaccharide and trisaccharide products.

AB - A synthetic pentasaccharide, containing an intact antithrombin III (ATIII) binding site that is in clinical studies a specific antifactor Xa agent, serves as a substrate for a heparin lyase (heparinase I, EC 4.2.2.7) from Flavobacterium heparinum. Heparinase I, currently being assessed as a heparin reversal agent, also reverses the antifactor Xa activity of this synthetic pentasaccharide by breaking it down to inactive disaccharide and trisaccharide products.

KW - Heparin lyase

KW - Heparinase

KW - Neutralization

KW - Pentasaccharide

KW - Reversal

UR - https://www.scopus.com/pages/publications/0034671656

U2 - 10.1016/S0049-3848(00)00368-6

DO - 10.1016/S0049-3848(00)00368-6

M3 - Article

C2 - 11152935

AN - SCOPUS:0034671656

SN - 0049-3848

VL - 100

SP - 549

EP - 556

JO - Thrombosis Research

JF - Thrombosis Research

IS - 6

ER -

Heparinase I acts on a synthetic heparin pentasaccharide corresponding to the antithrombin III binding site

Abstract

Keywords

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