Helicobacter pylori decreases gastric mucosal glutathione

Haim Shirin, John T. Pinto, Lawrence U. Liu, Mihai Merzianu, Emilia Mia Sordillo, Steven F. Moss

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Activation of oxidative stress pathways may contribute to gastric epithelial damage and mutagenesis caused by Helicobacter pylori. We measured the effect of H. pylori on the concentrations of reduced glutathione (GSH), an important endogenous defense against oxidant damage, in gastric epithelial cells in vivo and in vitro. GSH concentrations were significantly lower in gastric biopsies from 19 H. pylori-infected patients than 38 normal controls, and correlated inversely with inflammatory cell numbers. In vitro, H. pylori initially increased GSH levels in AGS cells, but subsequently depleted intracellular GSH stores completely after 24 h. No GSH was detected in H. pylori. Our data suggest that diminished GSH levels with H. pylori colonization of the gastric mucosa may be due to a direct effect of the bacterium as well as through the associated inflammatory response.

Original languageEnglish
Pages (from-to)127-133
Number of pages7
JournalCancer Letters
Volume164
Issue number2
DOIs
StatePublished - 26 Mar 2001
Externally publishedYes

Keywords

  • Carcinogenesis
  • Gastritis
  • Glutathione
  • Helicobacter pylori
  • Oxidative stress

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