TY - JOUR
T1 - Heart failure alters matrix metalloproteinase gene expression and activity in rat skeletal muscle
AU - Carvalho, Robson Francisco
AU - Dariolli, Rafael
AU - Justulin Junior, Luis Antonio
AU - Sugizaki, Mário Mateus
AU - Politi Okoshi, Marina
AU - Cicogna, Antonio Carlos
AU - Felisbino, Sérgio Luis
AU - Dal Pai-Silva, Maeli
PY - 2006/12
Y1 - 2006/12
N2 - Heart failure is associated with a skeletal muscle myopathy with cellular and extracellular alterations. The hypothesis of this investigation is that extracellular changes may be associated with enhanced mRNA expression and activity of matrix metalloproteinases (MMP). We examined MMP mRNA expression and MMP activity in Soleus (SOL), extensor digitorum longus (EDL), and diaphragm (DIA) muscles of young Wistar rat with monocrotaline-induced heart failure. Rats injected with saline served as age-matched controls. MMP2 and MMP9 mRNA contents were determined by RT-PCR and MMP activity by electrophoresis in gelatin-containing polyacrylamide gels in the presence of SDS under non-reducing conditions. Heart failure increased MMP9 mRNA expression and activity in SOL, EDL and DIA and MMP2 mRNA expression in DIA. These results suggest that MMP changes may contribute to the skeletal muscle myopathy during heart failure.
AB - Heart failure is associated with a skeletal muscle myopathy with cellular and extracellular alterations. The hypothesis of this investigation is that extracellular changes may be associated with enhanced mRNA expression and activity of matrix metalloproteinases (MMP). We examined MMP mRNA expression and MMP activity in Soleus (SOL), extensor digitorum longus (EDL), and diaphragm (DIA) muscles of young Wistar rat with monocrotaline-induced heart failure. Rats injected with saline served as age-matched controls. MMP2 and MMP9 mRNA contents were determined by RT-PCR and MMP activity by electrophoresis in gelatin-containing polyacrylamide gels in the presence of SDS under non-reducing conditions. Heart failure increased MMP9 mRNA expression and activity in SOL, EDL and DIA and MMP2 mRNA expression in DIA. These results suggest that MMP changes may contribute to the skeletal muscle myopathy during heart failure.
KW - Extracellular matrix
KW - Heart failure
KW - Metalloproteinases
KW - Skeletal muscle
KW - Wistar rats
UR - https://www.scopus.com/pages/publications/33751372451
U2 - 10.1111/j.1365-2613.2006.00497.x
DO - 10.1111/j.1365-2613.2006.00497.x
M3 - Article
C2 - 17222211
AN - SCOPUS:33751372451
SN - 0959-9673
VL - 87
SP - 437
EP - 443
JO - International Journal of Experimental Pathology
JF - International Journal of Experimental Pathology
IS - 6
ER -