@article{4c080ca1f5b94019b8f24f7b210276ba,
title = "Head-mounted microendoscopic calcium imaging in dorsal premotor cortex of behaving rhesus macaque",
abstract = "Microendoscopic calcium imaging with one-photon miniature microscopes enables unprecedented readout of neural circuit dynamics during active behavior in rodents. In this study, we describe successful application of this technology in the rhesus macaque, demonstrating plug-and-play, head-mounted recordings of cellular-resolution calcium dynamics from large populations of neurons simultaneously in bilateral dorsal premotor cortices during performance of a naturalistic motor reach task. Imaging is stable over several months, allowing us to longitudinally track individual neurons and monitor their relationship to motor behavior over time. We observe neuronal calcium dynamics selective for reach direction, which we could use to decode the animal's trial-by-trial motor behavior. This work establishes head-mounted microendoscopic calcium imaging in macaques as a powerful approach for studying the neural circuit mechanisms underlying complex and clinically relevant behaviors, and it promises to greatly advance our understanding of human brain function, as well as its dysfunction in neurological disease.",
keywords = "GCaMP, GRIN lens, arm reach, calcium imaging, decoding behavior, longitudinal tracking, macaque, microendoscopy, miniscope, premotor cortex",
author = "Anil Bollimunta and Santacruz, {Samantha R.} and Eaton, {Ryan W.} and Xu, {Pei S.} and Morrison, {John H.} and Moxon, {Karen A.} and Carmena, {Jose M.} and Nassi, {Jonathan J.}",
note = "Funding Information: This research was funded by a California National Primate Research Center (CNPRC) Pilot Research Program award to J.M.C., J.J.N., and K.A.M., and by National Institutes of Health /NINDS grant R01NS097480 and Bakar Fellow Funds from UC Berkeley to J.M.C. and a Defense Advanced Research Projects Agency (DARPA) Biological Technology Office (BTO) {\textquoteleft}{\textquoteleft}NeuroFAST{\textquoteright}{\textquoteright} Award W911NF-14-2-0013. We are grateful to Morrison lab member Sean Ott for virus preparation support and the CNPRC staff for support with behavioral training, surgery, pathology, and general care of animals. We thank Inscopix staff, including Arash Tajik, Sam Malanowski, Evan DeJarnette, and Mark Trulson, for supporting design, manufacturing, and testing of custom imaging components and David Cheng for testing of viruses. We thank Moxon lab members Ashley Schnider and Megan Santos for support with offline tracking of reaching behavior. We appreciate the helpful comments on the manuscript form Drs. Krishna Shenoy, Alice Stamatakis, Mark Miller, and Shay Neufeld. Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = jun,
day = "15",
doi = "10.1016/j.celrep.2021.109239",
language = "English",
volume = "35",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "11",
}