TY - JOUR
T1 - Guidelines for evaluating myocardial cell death
AU - Mishra, Paras K.
AU - Adameova, Adriana
AU - Hill, Joseph A.
AU - Baines, Christopher P.
AU - Kang, Peter M.
AU - Downey, James M.
AU - Narula, Jagat
AU - Takahashi, Masafumi
AU - Abbate, Antonio
AU - Piristine, Hande C.
AU - Kar, Sumit
AU - Su, Shi
AU - Higa, Jason K.
AU - Kawasaki, Nicholas K.
AU - Matsui, Takashi
N1 - Publisher Copyright:
Copyright © 2019 the American Physiological Society.
PY - 2019
Y1 - 2019
N2 - Cell death is a fundamental process in cardiac pathologies. Recent studies have revealed multiple forms of cell death, and several of them have been demonstrated to underlie adverse cardiac remodeling and heart failure. With the expansion in the area of myocardial cell death and increasing concerns over rigor and reproducibility, it is important and timely to set a guideline for the best practices of evaluating myocardial cell death. There are six major forms of regulated cell death observed in cardiac pathologies, namely apoptosis, necroptosis, mitochondrial-mediated necrosis, pyroptosis, ferroptosis, and autophagic cell death. In this article, we describe the best methods to identify, measure, and evaluate these modes of myocardial cell death. In addition, we discuss the limitations of currently practiced myocardial cell death mechanisms. Listen to this article's corresponding podcast at https://ajpheart.podbean.com/e/ guidelines-for-evaluating-myocardial-cell-death/.
AB - Cell death is a fundamental process in cardiac pathologies. Recent studies have revealed multiple forms of cell death, and several of them have been demonstrated to underlie adverse cardiac remodeling and heart failure. With the expansion in the area of myocardial cell death and increasing concerns over rigor and reproducibility, it is important and timely to set a guideline for the best practices of evaluating myocardial cell death. There are six major forms of regulated cell death observed in cardiac pathologies, namely apoptosis, necroptosis, mitochondrial-mediated necrosis, pyroptosis, ferroptosis, and autophagic cell death. In this article, we describe the best methods to identify, measure, and evaluate these modes of myocardial cell death. In addition, we discuss the limitations of currently practiced myocardial cell death mechanisms. Listen to this article's corresponding podcast at https://ajpheart.podbean.com/e/ guidelines-for-evaluating-myocardial-cell-death/.
KW - Apoptosis
KW - Autophagic cell death
KW - Cardiovascular disease
KW - Ferroptosis
KW - Heart
KW - Mitochondrial-mediated necrosis
KW - Necroptosis
KW - Pyroptosis
UR - http://www.scopus.com/inward/record.url?scp=85073764750&partnerID=8YFLogxK
U2 - 10.1152/AJPHEART.00259.2019
DO - 10.1152/AJPHEART.00259.2019
M3 - Review article
C2 - 31418596
AN - SCOPUS:85073764750
SN - 0363-6135
VL - 317
SP - H891-H922
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
IS - 5
ER -